Department of Life and Environmental Physics, "Horia Hulubei" National Institute of Physics and Nuclear Engineering, Măgurele, Romania.
Nosocomial and Antimicrobial Resistant Infections Laboratory, "Cantacuzino" National Medico-Military Institute for Research and Development, Bucharest, Romania.
Sci Rep. 2019 Nov 20;9(1):17176. doi: 10.1038/s41598-019-53926-4.
In recent years methicillin-resistant Staphylococcus aureus has posed a challenge in treating skin and soft tissue infections. Finding new antimicrobial agents has therefore become imperative. We evaluated the in vitro antimicrobial activity of a synthetic peptide, P6, against multidrug resistant clinical strains of Staphylococcus aureus isolated from skin and soft tissue infections. The P6 antimicrobial effect was evaluated in vitro by determining MIC/MBC, the ratio of live/dead cells and the effects induced at membrane level. The therapeutic efficiency was determined against human skin cells. P6 inhibited growth for all strains between 8 and 16 mg/L and killed all bacterial strains at 16 mg/L. The therapeutic potential was found to be 30 and 15 in the presence of BSA. We showed that P6 localizes at membrane level, where it acts slowly, by depolarizing it and affecting its integrity. P6 can be considered a good candidate for use as an antimicrobial agent in topical applications.
近年来，耐甲氧西林金黄色葡萄球菌对治疗皮肤和软组织感染构成了挑战。因此，寻找新的抗菌药物已变得势在必行。我们评估了一种合成肽 P6 对从皮肤和软组织感染分离的多药耐药临床金黄色葡萄球菌菌株的体外抗菌活性。通过测定 MIC/MBC、活/死细胞的比例以及在膜水平上诱导的作用，评估 P6 的抗菌效果。针对人皮肤细胞测定了治疗效率。P6 在 8 至 16mg/L 之间抑制所有菌株的生长，在 16mg/L 时杀死所有细菌菌株。在 BSA 存在的情况下，治疗潜力分别为 30 和 15。我们表明 P6 定位于膜水平，通过去极化和影响其完整性，其作用缓慢。P6 可被视为局部应用的一种有前途的抗菌剂候选药物。
