School of Chemistry and Chemical Engineering, Southeast University, Sipailou 2#, Nanjing 211189, PR China.
Talanta. 2010 Sep 15;82(4):1164-9. doi: 10.1016/j.talanta.2010.06.036. Epub 2010 Jul 3.
We have successfully constructed a novel gold film with open interconnected macroporous walls of nanoparticles by combining the hydrogen bubble dynamic template synthesis with galvanic replacement reaction. After modified by a self-assembled monolayer (SAM) of 11-mercaptoundecanoic acid (MUA), the three-dimensionally (3D) interconnected macroporous Au film has been used as a biocompatible substrate for the immobilization of cytochrome c. The morphology, structure and electrochemical features of the modified and unmodified macroporous Au films were characterized by field-emission scanning electron microscopy (FESEM), energy-dispersive X-ray (EDX), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The results reveal that the resultant films had a large electroactive surface area for high protein loading, enhanced electron transfer of cytochrome c, retained electrochemical activity, good stability and repeatability. And the excellent electrochemical behaviors could be attributed to the hierarchical structure of the macroporous Au film constructed by nanoparticles.
我们通过将氢气泡动态模板合成与电置换反应相结合，成功构建了一种具有开放互联纳米粒子大孔壁的新型金膜。经过 11-巯基十一酸（MUA）自组装单分子层修饰后，三维（3D）互联的大孔 Au 膜已被用作固定细胞色素 c 的生物相容基底。通过场发射扫描电子显微镜（FESEM）、能谱（EDX）、循环伏安法（CV）和电化学阻抗谱（EIS）对修饰和未修饰的大孔 Au 膜的形貌、结构和电化学特性进行了表征。结果表明，所得薄膜具有用于高蛋白质负载的大有效表面积、增强的细胞色素 c 的电子转移、保留的电化学活性、良好的稳定性和重现性。而优异的电化学行为归因于由纳米粒子构建的大孔 Au 膜的分级结构。
