Lancaster Environment Centre, Lancaster University, United Kingdom.
Environ Pollut. 2010 Nov;158(11):3411-20. doi: 10.1016/j.envpol.2010.07.034.
The influence of PAH chemical structure and concentration, added in either single (75 or 300 mg kg(-1)) or multiple (2 × 75, 2 × 150 or 4 × 75 mg kg(-1)) applications as single- or multiple-contaminant systems, on the development of PAH biodegradation in a pristine soil was investigated. Development in microbial catabolic ability was assessed at 0, 28, 56 and 84 d by monitoring (14)C-naphthalene, (14)C-phenanthrene and (14)C-pyrene mineralisation over 14 d in respirometric assays. The presence of other contaminants influenced the ability of the indigenous microflora to mineralise structurally different contaminants over time. (14)C-Naphthalene mineralisation was inhibited by the presence of other contaminants; whereas the presence of naphthalene significantly enhanced rates of mineralisation in multiple-contaminant systems containing (14)C-phenanthrene and (14)C-pyrene. Generally, increasing the number of contaminant applications has implications for catabolic activity of soil microbes. It is suggested the toxic nature of PAHs retarded mineralisation at increased contaminant concentrations.
研究了 PAH 化学结构和浓度（单一添加量为 75 或 300mg/kg，或双重添加量为 2×75、2×150 或 4×75mg/kg，作为单一或多重污染物体系）对原始土壤中 PAH 生物降解的影响。通过在呼吸测定中监测 14C-萘、14C-菲和 14C-芘在 14 天内的矿化作用，在 0、28、56 和 84d 评估微生物代谢能力的发展。其他污染物的存在影响了土著微生物群落随时间矿化结构不同污染物的能力。其他污染物的存在抑制了 14C-萘的矿化；而萘的存在显著提高了含有 14C-菲和 14C-芘的多重污染物体系中 14C-菲和 14C-芘的矿化速率。一般来说，增加污染物的应用次数会影响土壤微生物的代谢活性。据认为，PAHs 的毒性会减缓污染物浓度增加时的矿化作用。
