Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated Hospital, Harbin Medical University, Harbin 150081, China.
Eur J Cancer. 2010 Dec;46(18):3409-16. doi: 10.1016/j.ejca.2010.07.047.
MiRNAs are small, noncoding RNA molecules that emerge as important regulators of cancer-related processes. The miR-21 microRNA is overexpressed in a wide variety of cancers and has been causally related to cellular proliferation and apoptosis. In this study, we found that miR-21 is overexpressed in Laryngeal squamous cell carcinoma (LSCC) and correlated with advanced stage. Inhibition of miR-21 by antisense oligonucleotides (ASO) led to decreased protein level of Ras and profound suppression of cell proliferation and invasion. Hep-2 cells exposed to miR-21 ASO exhibited cell cycle arrest at G1 phase and increased apoptosis. Furthermore, growth of LSCC xenograft tumours was significantly suppressed by repeated injection of ASO-miR-21 lentivirus and the Ras protein expression in LSCC xenograft tumours was also downregulate by ASO-miR-21. Taken together, our data suggest that miR-21 may play an oncogenic role in the cellular processes of LSCC and represent a novel target for effective therapies.
miRNAs 是小的、非编码 RNA 分子，作为癌症相关过程的重要调节剂出现。miR-21 微 RNA 在多种癌症中过度表达，并与细胞增殖和凋亡有关。在这项研究中，我们发现 miR-21 在喉鳞状细胞癌 (LSCC) 中过度表达，并与晚期相关。反义寡核苷酸 (ASO) 抑制 miR-21 导致 Ras 蛋白水平降低，并显著抑制细胞增殖和侵袭。暴露于 miR-21 ASO 的 Hep-2 细胞在 G1 期停滞并增加凋亡。此外，重复注射 ASO-miR-21 慢病毒显著抑制 LSCC 异种移植瘤的生长，ASO-miR-21 还下调 LSCC 异种移植瘤中的 Ras 蛋白表达。总之，我们的数据表明，miR-21 可能在 LSCC 的细胞过程中发挥致癌作用，并代表有效治疗的新靶标。
