Department of Otorhinolaryngology, Head and Neck Surgery, The First Affiliated Hospital, Harbin Medical University, Harbin 150081, PR China.
Anticancer Res. 2011 Nov;31(11):3859-63.
MicroRNAs (miRNA) are a class of small noncoding RNAs that regulate the expression of their target genes. The aim of the present study was to explore the effects of miR-206 on laryngeal suamous cell carcinoma (LSCC) cells. The expression level of miR-206 was quantified by qRT-PCR in primary LSCC tissues and corresponding adjacent non-neoplastic tissues. MTT, Matrigel invasion assays and flow cytometry methods were used to test the proliferation, invasion and apoptosis of MiR-206 transfection LSCC cells and a mouse model was used to investigate tumorigenesis. MiR-206 was significantly down regulated in the LSCC tissues. Inverse correlation of miR-206 expression was found with the T grade, nodal metastasis and clinical stage of LSCC. Cell proliferation, migration, invasion and tumorigenesis in the LSCC cells were significantly inhibited and apoptotic cells were also increased after miR-206 tansfection. Furthermore, miR-206 transfection down-regulated the expression of vascular endothelial growth factor (VEGF) in the LSCC cells. The loss of miR-206 may play an important role in the progress of LSCC and miR-206 may function as a novel tumor suppressed miRNA.
微小 RNA（miRNA）是一类小的非编码 RNA，可调节其靶基因的表达。本研究旨在探讨 miR-206 对喉鳞状细胞癌（LSCC）细胞的影响。通过 qRT-PCR 定量检测 miR-206 在原发性 LSCC 组织和相应的相邻非肿瘤组织中的表达水平。MTT、Matrigel 侵袭实验和流式细胞术方法用于检测 miR-206 转染 LSCC 细胞的增殖、侵袭和凋亡，并用小鼠模型研究肿瘤发生。miR-206 在 LSCC 组织中明显下调。miR-206 的表达与 LSCC 的 T 分级、淋巴结转移和临床分期呈负相关。miR-206 转染后 LSCC 细胞的增殖、迁移、侵袭和肿瘤发生明显受到抑制，凋亡细胞也增加。此外，miR-206 转染下调了 LSCC 细胞中血管内皮生长因子（VEGF）的表达。miR-206 的缺失可能在 LSCC 的进展中发挥重要作用，miR-206 可能作为一种新的肿瘤抑制 miRNA 发挥作用。
