Department of Radiology, University of Minnesota Medical Center MMC 292, 420 Delaware Street SE, Minneapolis, MN 55455, USA.
J Vasc Interv Radiol. 2010 Oct;21(10):1573-8. doi: 10.1016/j.jvir.2010.06.012.
To establish proof of concept in tissue, using the exothermic neutralization reaction of acetic acid and sodium hydroxide in ex vivo porcine liver and to conduct an initial probe into the relationships of volume and concentration of reagents to temperatures and the areas affected.
A total of 0.5 mL or 2 mL of either 5 mole/L or 10 mole/L acid and base solutions was injected simultaneously into the periphery of ex vivo porcine liver using a prototype injection device. Tissue temperature was recorded at the injection site for 5 minutes using a type T thermocouple temperature probe inserted parallel to and near the tip of the injection device. The injections were repeated for infrared thermography, and ablated tissues were sectioned quickly and imaged. A gross photograph was captured in each case to provide correlation.
Maximum temperatures (17°C baseline) ranged from 42.1° ± α3.34°C to 61.7° ± α10°C (P<.05) when injecting 0.5 mL of 5 mole/L reactants and 2 mL of 10 mole/L reactants, respectively. The maximum temperature measured by infrared imaging ranged from 31°-47°C. Using an infrared viewing scale from 19°-40°C, the cross-sectional area of tissue heating above baseline measured from 1.07 cm(2)± 0.45 to 4.95 cm(2)± 0.28 (P <05).
The reaction of acetic acid and sodium hydroxide releases significant heat energy at the site of injection, and histologic changes are consistent with coagulation necrosis. Increased reagent concentration and volume were associated with larger temperature changes and larger areas of hyperthermia at gross pathology and infrared imaging.
利用乙酸和氢氧化钠在离体猪肝中的放热中和反应在组织中建立概念验证，并初步探讨试剂体积和浓度与温度和受影响区域的关系。
使用原型注射装置将 0.5 毫升或 2 毫升的 5 摩尔/升或 10 摩尔/升的酸和碱溶液同时注入离体猪肝的外围。使用插入与注射装置尖端平行且靠近的 T 型热电偶温度探头在注射部位记录组织温度 5 分钟。为了进行红外热成像，重复注射，并快速切除和成像消融组织。在每种情况下都拍摄了大体照片以提供相关性。
当注射 0.5 毫升 5 摩尔/升反应物和 2 毫升 10 摩尔/升反应物时，最大温度（17°C 基线）范围从 42.1°±α3.34°C 到 61.7°±α10°C（P<.05）。红外成像测量的最大温度范围为 31°-47°C。使用 19°-40°C 的红外观察刻度，从基线以上测量的组织加热横截面面积从 1.07 cm²±0.45 增加到 4.95 cm²±0.28（P <05）。
乙酸和氢氧化钠的反应在注射部位释放出大量的热能，组织学变化与凝固性坏死一致。增加试剂浓度和体积与更大的温度变化和更大的大体病理学和红外成像上的过热区域相关。
