Department of Microbiology & Immunology, JamesBrown Cancer Center, University of Louisville, Louisville, Kentucky 40202, USA.
Am J Pathol. 2010 Oct;177(4):1606-10. doi: 10.2353/ajpath.2010.100245. Epub 2010 Aug 27.
Exosomes released from tumor cells having been shown to induce interleukin-6 release from myeloid-derived suppressor cells in a Toll-like receptor 2/Stat3-dependent manner. In this study, we show that exosomes released from tumor cells re-isolated from syngeneic mice are capable of inducing interleukin-6 in a Toll-like receptor 2-independent manner, whereas the data generated from exosomes of tumor cells having undergone numerous in vitro passages induce interleukin-6 in a Toll-like receptor 2-dependent manner. This discrepancy may be due to the source of tumor cells used to generate the exosomes for this study. These results suggest that exosomes released from tumor cells that are not within a tumor microenvironment may not realistically represent the role of tumor exosomes in vivo. This is an important consideration since frequently passing tumor cells in vivo is an accepted practice for studying tumor exosome-mediated inflammatory responses.
已证实肿瘤细胞释放的外泌体能够通过 Toll 样受体 2/Stat3 依赖性方式诱导髓源抑制细胞释放白细胞介素-6。在这项研究中，我们表明，从同种小鼠中重新分离的肿瘤细胞释放的外泌体能够以 Toll 样受体 2 非依赖性方式诱导白细胞介素-6，而来自经历了多次体外传代的肿瘤细胞的外泌体则以 Toll 样受体 2 依赖性方式诱导白细胞介素-6。这种差异可能是由于用于生成本研究中外泌体的肿瘤细胞来源不同。这些结果表明，来自肿瘤微环境之外的肿瘤细胞释放的外泌体可能无法真实地代表肿瘤外泌体在体内的作用。这是一个重要的考虑因素，因为经常在体内传递肿瘤细胞是研究肿瘤外泌体介导的炎症反应的一种被接受的做法。
