Laranjeira Vani Dos Santos, Marchetti Desiree Padilha, Steyer Juçara Rodrigues, Corção Gertrudes, Picoli Simone Ulrich
Instituto de Ciências da Saúde, Universidade Feevale, Novo Hamburgo, RS.
Rev Soc Bras Med Trop. 2010 Jul-Aug;43(4):462-4. doi: 10.1590/s0037-86822010000400026.
The appearance of metallo-beta-lactamase (MBL)-producing Pseudomonas aeruginosa and Acinetobacter sp. is a challenge for hospitals.
The production of MBL in clinical isolates of Pseudomonas aeruginosa and Acinetobacter sp. From an emergency hospital in Porto Alegre was investigated using the disk approximation test and MBL E-test. The bla genes were determined using PCR.
Two strains of Pseudomonas aeruginosa and eight of Acinetobacter sp were shown to be MBL phenotypes. Amplification of the blaSPM-1 gene confirmed the presence of the enzyme in P. aeruginosa.
Caution is needed in evaluating phenotype tests used for routine detection of metallo-beta-lactamases.
产金属β-内酰胺酶（MBL）的铜绿假单胞菌和不动杆菌属的出现对医院构成了挑战。
采用纸片扩散法和MBL E试验，对阿雷格里港一家急诊医院临床分离的铜绿假单胞菌和不动杆菌属菌株进行MBL检测。采用聚合酶链反应（PCR）检测bla基因。
两株铜绿假单胞菌和八株不动杆菌属菌株表现为MBL表型。blaSPM-1基因扩增证实铜绿假单胞菌中存在该酶。
在评估用于常规检测金属β-内酰胺酶的表型试验时需谨慎。
