Molecular Cell Physiology, Bielefeld University, 33501, Bielefeld, Germany.
Cell Mol Life Sci. 2011 Jan;68(1):71-83. doi: 10.1007/s00018-010-0513-5. Epub 2010 Aug 30.
An endogenous timing mechanism, the circadian clock, causes rhythmic expression of a considerable fraction of the genome of most organisms to optimally align physiology and behavior with their environment. Circadian clocks are self-sustained oscillators primarily based on transcriptional feedback loops and post-translational modification of clock proteins. It is increasingly becoming clear that regulation at the RNA level strongly impacts the cellular circadian transcriptome and proteome as well as the oscillator mechanism itself. This review focuses on posttranscriptional events, discussing RNA-binding proteins that, by influencing the timing of pre-mRNA splicing, polyadenylation and RNA decay, shape rhythmic expression profiles. Furthermore, recent findings on the contribution of microRNAs to orchestrating circadian rhythms are summarized.
内源性时间机制——生物钟——使大多数生物的大部分基因组以有节奏的方式表达,从而使生理和行为与环境最佳匹配。生物钟主要基于转录反馈回路和时钟蛋白的翻译后修饰,是自我维持的振荡器。越来越明显的是,RNA 水平的调节强烈影响细胞生物钟的转录组和蛋白质组以及振荡器机制本身。本文重点讨论了转录后事件,讨论了 RNA 结合蛋白,它们通过影响前体 mRNA 剪接、多聚腺苷酸化和 RNA 降解的时间,形成有节奏的表达谱。此外,还总结了最近关于 microRNAs 对协调生物钟节律的贡献的发现。