Hallegot P, Girod C, Levi-Setti R
Enrico Fermi Institute, University of Chicago, Illinois 60637.
Scanning Microsc. 1990 Sep;4(3):605-12.
Different preparation techniques for high lateral resolution scanning ion microprobe imaging of biological samples have been investigated. The sharpest histological maps are obtained from chemically fixed and plastic embedded specimens. It is often problematic to correlate ultrastructure and bioaccumulation from analysis of frozen cut and lyophilized sections. The best compromise is to resin-embed frozen samples in order to get a perfectly flat section from tissue where the in vivo ion distribution is maintained. Use of the University of Chicago Ion Microprobe gave us the ability to observe the relative ion translocations induced during sample preparation. As an example, we show the rapid decrease of intracellular K+/Na+ ratio through a fast frozen blood droplet.
已经研究了用于生物样品高横向分辨率扫描离子微探针成像的不同制备技术。最清晰的组织学图谱是从化学固定和塑料包埋的标本中获得的。通过对冷冻切片和冻干切片的分析来关联超微结构和生物累积往往存在问题。最佳折衷方案是将冷冻样品树脂包埋，以便从保持体内离子分布的组织中获得完美平整的切片。使用芝加哥大学离子微探针使我们能够观察样品制备过程中诱导的相对离子转运。例如，我们展示了通过快速冷冻血滴，细胞内K+/Na+比率的快速下降。
