DNA聚合酶α与复制中的SV40核蛋白复合物相关联。
DNA polymerase alpha is associated with replicating SV40 nucleoprotein complexes.
作者信息
Otto B, Fanning E
出版信息
Nucleic Acids Res. 1978 May;5(5):1715-28. doi: 10.1093/nar/5.5.1715.
Abstract
Simian virus 40 (SV40) nucleoprotein complexes were extracted from nuclei of infected monkey cells and fractionated on neutral sucrose density gradients. Complexes which contained replicating SV40 DNA (95S) separated well from those containing closed circular supercoiled viral DNA (75S). DNA polymerase activity was associated with the replicating nucleoprotein complexes but not with the slower sedimenting complexes. This DNA polymerase activity coprecipitated with the nucleoprotein complexes in the presence of MgCl2 and remained associated with the 95S complexes. This DNA polymerase activity has been identified as primarily DNA polymerase alpha on the basis of its sedimentation behavior, optimum salt concentration, and sensitivity to N-ethylmaleimide. DNA polymerase gamma activity was also detected in the complexes, but DNA polymerase beta was not associated with the complexes.
摘要
从感染猴细胞的细胞核中提取猴病毒40（SV40）核蛋白复合物，并在中性蔗糖密度梯度上进行分级分离。含有正在复制的SV40 DNA（95S）的复合物与含有闭环超螺旋病毒DNA（75S）的复合物分离良好。DNA聚合酶活性与正在复制的核蛋白复合物相关，而与沉降较慢的复合物无关。在MgCl2存在下，这种DNA聚合酶活性与核蛋白复合物共沉淀，并与95S复合物保持关联。根据其沉降行为、最佳盐浓度和对N-乙基马来酰亚胺的敏感性，这种DNA聚合酶活性已被确定主要为DNA聚合酶α。在复合物中也检测到了DNA聚合酶γ活性，但DNA聚合酶β与复合物无关。
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