Manganese-enhanced MRI of salivary glands and head and neck tumors in living subjects.

Manganese-enhanced MRI has previously been used for visualization of brain architecture and functional mapping of neural pathways. The present work investigated the potential of manganese-enhanced MRI for noninvasive imaging of salivary glands in living subjects. Marked shortening of T(1) was observed in salivary glands of naïve mice (n = 5) 24-48 h after systemic administration of MnCl(2) (0.4 mmol/kg, intraperitoneally). Three-dimensional MR microscopy confirmed selective contrast enhancement of salivary gland tissues post-MnCl(2) injection. Ectopic and orthotopic head and neck tumor xenografts also showed an increase in R(1) at 24 h following MnCl(2) injection (0.2 mmol/kg, intraperitoneally). However, tumor enhancement was minimal compared to salivary gland tissue. Salivary gland R(1) values were lower in mice bearing orthotopic head and neck tumors compared to naïve mice. These results demonstrate, for the first time, the usefulness of manganese-enhanced MRI in the visualization of salivary glands and head and neck tumors in vivo.