Dahlstrøm Berit, Esbensen Ying, Vollan Hilde, Oian Pål, Bukholm Geir
Department of Obstetrics and Gynecology, Faculty Division Akershus University Hospital, University of Oslo, N-1474 Nordbyhagen, Norway.
J Perinat Med. 2010 Nov;38(6):601-8. doi: 10.1515/jpm.2010.095. Epub 2010 Aug 31.
inflammatory processes are present during preeclampsia and in normal pregnancy. Maternal inflammatory reactions may change towards term. Our objective was to evaluate genome signaling in blood during preeclampsia and towards term using microarrays.
RNA microarrays (Illumina) were conducted on blood from preeclamptic pregnancies delivered preterm, normal pregnancies at term and normal pregnancies at gestational week 31. Two statistical methods (Q-value cut-off 1%) identified data structures in the three groups and retrieved activated genes along a time axis and a diseased-healthy axis. Signaling genes were localized within known pathways and gene sets, and genes associated with inflammation were identified.
early onset preeclampsia and term pregnancies both showed distinct expression patterns when compared to normal pregnancy at gestational week 31. In preeclampsia, 19 genes were differentially expressed, including a down-regulation of CC-chemokine receptor 3 (CCR3). Among the 183 differentially expressed genes towards term, tumor necrosis factor superfamily member 15 (TNFSF15) was up-regulated and interferon-γ receptor 2 (IFNGR2) and CXC-chemokine receptor type 4 (CXCR4) were down-regulated. Seven of the genes were similarly changed during preeclampsia and towards term.
a possible type 1 immune response was identified both during preeclampsia and towards term. In pre-eclampsia a premature activation of leucocytes might be present.
子痫前期和正常妊娠过程中均存在炎症过程。母体炎症反应可能在孕晚期发生变化。我们的目的是使用微阵列评估子痫前期及孕晚期血液中的基因组信号。
对早产的子痫前期妊娠、足月正常妊娠及妊娠31周的正常妊娠的血液进行RNA微阵列（Illumina）检测。两种统计方法（Q值截断为1%）确定了三组中的数据结构，并沿着时间轴和疾病-健康轴检索了激活的基因。将信号基因定位在已知的通路和基因集中，并鉴定与炎症相关的基因。
与妊娠31周的正常妊娠相比，早发型子痫前期和足月妊娠均表现出独特的表达模式。子痫前期中有19个基因差异表达，包括CC趋化因子受体3（CCR3）的下调。在孕晚期差异表达的183个基因中，肿瘤坏死因子超家族成员15（TNFSF15）上调，干扰素-γ受体2（IFNGR2）和CXC趋化因子受体4（CXCR4）下调。其中7个基因在子痫前期和孕晚期的变化相似。
子痫前期及孕晚期均识别出可能的1型免疫反应。子痫前期可能存在白细胞的过早激活。
