Garon Catherine L, Cohn Leah A, Scott Michael A
Department of Veterinary Medicine and Surgery, College of Veterinary Medicine, University of Missouri, Columbia, MO 65211, USA.
Am J Vet Res. 2010 Sep;71(9):1033-8. doi: 10.2460/ajvr.71.9.1033.
To determine erythrocyte survival time in Greyhounds.
6 Greyhounds used as blood donors and 3 privately owned non-Greyhound dogs.
In vivo biotinylation of erythrocytes was performed by infusion of biotin-N-hydroxysuccinimide into each dog via a jugular vein catheter. Blood samples were collected 12 hours later and then at weekly intervals and were used to determine the percentage of biotin-labeled erythrocytes at each time point. Erythrocytes were washed, incubated with avidin-fluorescein isothiocyanate, and washed again before the percentage of biotinylated erythrocytes was measured by use of flow cytometry. Survival curves for the percentage of biotinylated erythrocytes were generated, and erythrocyte survival time was defined as the x-intercept of a least squares best-fit line for the linear portion of each curve.
The R2 for survival curves ranged from 0.93 to 0.99 during the first 10 weeks after infusion of erythrocytes. Erythrocyte survival time for the 3 non-Greyhound dogs was 94, 98, and 116 days, respectively, which was consistent with previously reported values. Erythrocyte survival time for the 6 Greyhounds ranged from 83 to 110 days (mean, 93 days; median, 88 days). As determined by use of in vivo biotinylation, erythrocyte survival times in Greyhounds were similar to those determined for non-Greyhound dogs and did not differ significantly from erythrocyte survival times reported previously for non-Greyhound dogs.
Erythrocyte survival time was similar in Greyhounds and non-Greyhound dogs. Greyhounds can be used as erythrocyte donors without concerns about inherently shorter erythrocyte survival time.
测定灵缇犬的红细胞存活时间。
6只用作供血者的灵缇犬和3只 privately owned非灵缇犬。
通过颈静脉导管向每只犬输注生物素 - N - 羟基琥珀酰亚胺，对红细胞进行体内生物素化。12小时后采集血样，随后每周采集一次，用于测定每个时间点生物素标记红细胞的百分比。红细胞经洗涤后，与抗生物素蛋白 - 异硫氰酸荧光素孵育，再次洗涤后，使用流式细胞术测量生物素化红细胞的百分比。生成生物素化红细胞百分比的存活曲线，红细胞存活时间定义为每条曲线线性部分最小二乘最佳拟合线的x轴截距。
输注红细胞后的前10周内，存活曲线的R2范围为0.93至0.99。3只非灵缇犬的红细胞存活时间分别为94天、98天和116天，与先前报道的值一致。6只灵缇犬的红细胞存活时间为83至110天（平均93天；中位数88天）。通过体内生物素化测定，灵缇犬的红细胞存活时间与非灵缇犬相似，与先前报道的非灵缇犬红细胞存活时间无显著差异。
灵缇犬和非灵缇犬的红细胞存活时间相似。灵缇犬可作为红细胞供体，无需担心其红细胞存活时间固有地较短。
