Novinger M S, Sullivan P S, McDonald T P
Department of Animal Science, College of Veterinary Medicine, University of Tennessee, Knoxville 37901-1071, USA.
Am J Vet Res. 1996 May;57(5):739-42.
To determine the RBC lifespan of Greyhounds, using an in vitro labeling technique.
RBC from dogs were labeled with NHS-biotin and their disappearance measured over time to determine RBC lifespan.
5 Greyhounds that had been vaccinated against distemper, adenovirus 1 and 2 infections, parainfluenza, leptospirosis, parvovirus, and coronavirus infections, Bordetella bronchiseptica infection, and rabies the previous year; 3 sexually intact 14-month-old Beagles served as controls.
After venipuncture for CBC, catheters were inserted in the cephalic vein of each dog. Butorphanol was then administered to achieve mild sedation and analgesia, and glycopyrrolate was administered to ensure maintenance of adequate heart rate during phlebotomy. Dogs were positioned in lateral recumbency; blood was removed via jugular venipuncture, using a standard laboratory donor blood bag containing citrate-phosphate-dextrose solution. Blood was transferred aseptically into sterile polystyrene containers and NHS-biotin was added. After incubation, the labeled RBC were reinfused into the dogs and the blood was allowed to recirculate for 1 hour before the first postinfusion sample was taken. At frequent intervals, blood to be analyzed was taken by jugular venipuncture, and the percentage of labeled cells was determined by flow cytometry.
The mean RBC lifespan of non-Greyhounds was significantly longer than that of Greyhounds (104.3 +/- 2.2 days vs 53.6 +/- 6.5 days; P = 0.001). A negative linear correlation was also found between age of the Greyhounds and their RBC lifespan (P = 0.01, R2 = 0.91).
The shorter RBC lifespan of the Greyhounds may explain the finding of macrocytosis reported in earlier work. The reason for the shorter RBC lifespan in Greyhounds may be caused by differences in Greyhound RBC membrane structure or accelerated RBC removal from the circulation.
采用体外标记技术测定灵缇犬的红细胞寿命。
用NHS-生物素标记犬的红细胞，并随时间测量其消失情况以确定红细胞寿命。
5只去年已接种犬瘟热、腺病毒1型和2型感染、副流感、钩端螺旋体病、细小病毒和冠状病毒感染、支气管败血波氏杆菌感染及狂犬病疫苗的灵缇犬；3只性成熟的14月龄比格犬作为对照。
静脉穿刺进行血常规检查后，在每只犬的头静脉插入导管。然后给予布托啡诺以达到轻度镇静和镇痛效果，并给予格隆溴铵以确保在放血过程中维持足够的心率。犬侧卧；通过颈静脉穿刺采血，使用装有枸橼酸盐-磷酸盐-葡萄糖溶液的标准实验室供血袋。血液无菌转移至无菌聚苯乙烯容器中并加入NHS-生物素。孵育后，将标记的红细胞重新注入犬体内，让血液再循环1小时后采集首次输注后样本。每隔一段时间，通过颈静脉穿刺采集待分析的血液，并用流式细胞术测定标记细胞的百分比。
非灵缇犬的平均红细胞寿命显著长于灵缇犬（104.3±2.2天对53.6±6.5天；P = 0.001）。还发现灵缇犬的年龄与其红细胞寿命呈负线性相关（P = 0.01，R2 = 0.91）。
灵缇犬较短的红细胞寿命可能解释了早期研究中报道的大红细胞症这一发现。灵缇犬红细胞寿命较短的原因可能是灵缇犬红细胞膜结构的差异或循环中红细胞清除加速所致。
