Mudge Margaret C, Walker Naomi J, Borjesson Dori L, Librach Fred, Johns Jennifer L, Owens Sean D
Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus, OH, USA.
Vet Clin Pathol. 2012 Mar;41(1):56-62. doi: 10.1111/j.1939-165X.2011.00384.x. Epub 2012 Jan 17.
Post-transfusion survival of allogeneic RBCs has been reported to be much shorter in horses than in other species. We hypothesized that post-transfusion survival of biotinylated allogeneic equine RBCs would be greater than the survival previously reported from studies using radioactive RBC-labeling techniques.
The study objective was to determine post-transfusion survival of N-hydroxysuccinimide (NHS)-biotin-labeled allogeneic equine RBCs transfused into adult horses.
Horses were adults and included 5 donors and 5 recipients. All horses were blood-typed, and donors were paired with recipients based upon blood type and crossmatch results. Donor blood was collected in a volume of 4 L into citrate phosphate dextrose adenine-1 and stored for 24 hours, labeled with NHS-biotin, and re-infused into recipients. Post-transfusion blood samples were collected at 15 minutes and at 1, 2, 3, 5, 7, 14, 21, 28, and 35 days. Biotin-labeled RBCs were detected by flow cytometry using streptavidin-phycoerythrin. Post-transfusion survival at 24 hours, lifespan, and half-life of biotinylated RBCs were determined.
Mean ± SD survival of biotinylated RBCs at 24 hours post-transfusion was 95 ± 24%; the mean lifespan of transfused allogeneic RBCs was 39 days based on calculation of a linear regression survival curve, and mean post-transfusion RBC half-life was 20 days.
Post-transfusion survival of 24-hour stored equine allogeneic RBCs was greater than previously reported but less than that observed for other companion animal species. Mechanisms for the relatively short post-transfusion lifespan of allogeneic equine RBCs remain unknown and warrant further study.
据报道，同种异体红细胞在马体内的输血后存活时间比其他物种短得多。我们假设，生物素化的同种异体马红细胞的输血后存活时间会比先前使用放射性红细胞标记技术的研究所报道的存活时间更长。
本研究的目的是确定输注到成年马体内的N-羟基琥珀酰亚胺（NHS）-生物素标记的同种异体马红细胞的输血后存活时间。
实验对象为成年马，包括5匹供体马和5匹受体马。对所有马匹进行血型鉴定，并根据血型和交叉配血结果将供体与受体配对。采集4L供体血液，放入含枸橼酸盐、磷酸盐、葡萄糖和腺嘌呤-1的溶液中，储存24小时，用NHS-生物素标记，然后重新输注到受体体内。在输血后15分钟以及第1、2、3、5、7、14、21、28和35天采集输血后的血样。使用链霉亲和素-藻红蛋白通过流式细胞术检测生物素标记的红细胞。测定生物素化红细胞在输血后24小时的存活情况、寿命和半衰期。
输血后24小时，生物素化红细胞的平均存活时间±标准差为95±24%；根据线性回归存活曲线计算，输注的同种异体红细胞的平均寿命为39天，输血后红细胞的平均半衰期为20天。
储存24小时的马同种异体红细胞的输血后存活时间比先前报道的更长，但比其他伴侣动物物种观察到的存活时间短。同种异体马红细胞输血后寿命相对较短的机制尚不清楚，值得进一步研究。
