促胰液素、血管活性肠肽和生长抑素对培养脑细胞中环磷酸腺苷积累的调节作用。
Regulation by secretin, vasoactive intestinal peptide, and somatostatin of cyclic AMP accumulation in cultured brain cells.
作者信息
van Calker D, Müller M, Hamprecht B
出版信息
Proc Natl Acad Sci U S A. 1980 Nov;77(11):6907-11. doi: 10.1073/pnas.77.11.6907.
Abstract
Secretin stimulates the accumulation of cyclic AMP (half maximally stimulating concentration: 10-20 nM) in cultured mouse brain cells mainly consisting of glioblasts. Vasoactive intestinal peptide (VIP) is much less potent in raising the level of cyclic AMP in these cultures. The effect of secretin but not that of VIP is inhibited by secretin-(5-27), a synthetic antagonist of secretin. Stimulation of the adrenergic alpha-receptors and the adenosine A1-receptors present on the cells attenuates the increase in cyclic AMP evoked by secretin and VIP. Somatostatin at low concentrations inhibits the accumulation of cyclic AMP (half-maximally inhibitory concentration: 3 nM), in the absence or presence of secretin, VIP, or isoproterenol. The results suggest that secretin might regulate the concentration of cyclic AMP in brain and provoke the question of a possible involvement of glial cells in the action of peptide hormones in the brain.
摘要
促胰液素可刺激主要由成胶质细胞组成的培养小鼠脑细胞中环状AMP的积累（最大刺激浓度的一半：10 - 20 nM）。血管活性肠肽（VIP）在提高这些培养物中环状AMP水平方面的效力要低得多。促胰液素的作用而非VIP的作用可被促胰液素 -（5 - 27）（促胰液素的一种合成拮抗剂）抑制。刺激细胞上存在的肾上腺素能α受体和腺苷A1受体可减弱促胰液素和VIP引起的环状AMP增加。在不存在或存在促胰液素、VIP或异丙肾上腺素的情况下，低浓度的生长抑素会抑制环状AMP的积累（最大抑制浓度的一半：3 nM）。结果表明，促胰液素可能调节大脑中环状AMP的浓度，并引发了胶质细胞可能参与大脑中肽类激素作用的问题。
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