一种用于基质辅助激光解吸电离质谱（MALDI-MS）的准确且清洁的校准方法。
An accurate and clean calibration method for MALDI-MS.
作者信息
Xiang Bosong, Prado Mindy
机构信息
Monsanto Company, St. Louis, Missouri 63167, USA.
出版信息
J Biomol Tech. 2010 Sep;21(3):116-9.
Abstract
A new method for the mass calibration of the matrix-assisted laser desorption/ionization-mass spectrometry spectrum is introduced. This method achieves the same accuracy as that of internal calibration but without its drawbacks. The interference and signal suppression by calibration standard are avoided, and a pure/clean sample spectrum is obtained. No prior knowledge about the sample quantity is required for the calibration. The effectiveness of the method is demonstrated with protein identification data.
摘要
介绍了一种用于基质辅助激光解吸/电离质谱谱图质量校准的新方法。该方法实现了与内标校准相同的准确度，但没有其缺点。避免了校准标准物的干扰和信号抑制，获得了纯净的样品谱图。校准不需要关于样品量的先验知识。通过蛋白质鉴定数据证明了该方法的有效性。
相似文献
1
An accurate and clean calibration method for MALDI-MS.一种用于基质辅助激光解吸电离质谱（MALDI-MS）的准确且清洁的校准方法。
J Biomol Tech. 2010 Sep;21(3):116-9.
2
Closely spaced external standard: a universal method of achieving 5 ppm mass accuracy over the entire MALDI plate in axial matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.紧密间隔外部标准：一种在轴向基质辅助激光解吸/电离飞行时间质谱中在整个MALDI板上实现5 ppm质量精度的通用方法。
Rapid Commun Mass Spectrom. 2003;17(19):2177-87. doi: 10.1002/rcm.1158.
3
Matrix ions as internal standard for high mass accuracy matrix-assisted laser desorption/ionization mass spectrometry imaging.基质离子作为内标物用于高质量精度基质辅助激光解吸/电离质谱成像。
Rapid Commun Mass Spectrom. 2021 Aug 30;35(16):e9110. doi: 10.1002/rcm.9110.
4
The use of pencil lead as a matrix and calibrant for matrix-assisted laser desorption/ionisation.使用铅笔芯作为基质辅助激光解吸/电离的基质和校准物。
Rapid Commun Mass Spectrom. 2006;20(7):1053-60. doi: 10.1002/rcm.2408.
5
PAS-cal: A repetitive peptide sequence calibration standard for MALDI mass spectrometry.PAS-cal：一种用于基质辅助激光解吸电离质谱的重复肽序列校准标准品。
Proteomics. 2014 Nov;14(21-22):2427-31. doi: 10.1002/pmic.201400199. Epub 2014 Sep 25.
6
Characterization of poly(butylene terephthalate) by size-exclusion chromatography and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.采用尺寸排阻色谱法和基质辅助激光解吸/电离飞行时间质谱法对聚对苯二甲酸丁二醇酯进行表征。
J Chromatogr A. 2002 Nov 8;976(1-2):145-54. doi: 10.1016/s0021-9673(02)01149-4.
7
Precalibration of matrix-assisted laser desorption/ionization-time of flight spectra for peptide mass fingerprinting.用于肽质量指纹图谱分析的基质辅助激光解吸/电离飞行时间光谱的预校准
Proteomics. 2002 Oct;2(10):1365-73. doi: 10.1002/1615-9861(200210)2:10<1365::AID-PROT1365>3.0.CO;2-9.
8
Chemically-assisted fragmentation combined with multi-dimensional liquid chromatography and matrix-assisted laser desorption/ionization post source decay, matrix-assisted laser desorption/ionization tandem time-of flight or matrix-assisted laser desorption/ionization tandem mass spectrometry for improved sequencing of tryptic peptides.化学辅助碎裂结合多维液相色谱和基质辅助激光解吸/电离源后衰变、基质辅助激光解吸/电离串联飞行时间或基质辅助激光解吸/电离串联质谱用于改进胰蛋白酶肽段的测序。
Eur J Mass Spectrom (Chichester). 2005;11(2):169-79. doi: 10.1255/ejms.734.
9
Matrix-assisted laser desorption/ionization mass spectrometry of discrete mass poly(butylene glutarate) oligomers.离散质量聚丁二酸丁二醇酯低聚物的基质辅助激光解吸/电离质谱分析
Anal Chem. 2003 Jul 1;75(13):3092-100. doi: 10.1021/ac030061q.
10
Internal calibration on adjacent samples (InCAS) with Fourier transform mass spectrometry.傅里叶变换质谱法对相邻样本进行内部校准（InCAS）。
Anal Chem. 2000 Dec 15;72(24):5881-5. doi: 10.1021/ac000770t.
引用本文的文献
1
Mass shift in mass spectrometry imaging: comprehensive analysis and practical corrective workflow.质谱成像中的质量迁移：全面分析与实用校正工作流程。
Anal Bioanal Chem. 2021 Apr;413(10):2831-2844. doi: 10.1007/s00216-021-03174-1. Epub 2021 Jan 30.
2
Characterization of Synthetic Polymers via Matrix Assisted Laser Desorption Ionization Time of Flight (MALDI-TOF) Mass Spectrometry.通过基质辅助激光解吸电离飞行时间（MALDI-TOF）质谱法对合成聚合物进行表征。
J Vis Exp. 2018 Jun 10(136):57174. doi: 10.3791/57174.
3
Diagnosing and Correcting Mass Accuracy and Signal Intensity Error Due to Initial Ion Position Variations in a MALDI TOFMS.诊断并校正基质辅助激光解吸电离飞行时间质谱中由于初始离子位置变化导致的质量精度和信号强度误差。
J Am Soc Mass Spectrom. 2018 Feb;29(2):422-434. doi: 10.1007/s13361-017-1845-2. Epub 2017 Dec 7.
4
Optimizing sequence coverage for a moderate mass protein in nano-electrospray ionization quadrupole time-of-flight mass spectrometry.优化纳米电喷雾电离四极杆飞行时间质谱中中等质量蛋白质的序列覆盖度。
Anal Biochem. 2016 Sep 15;509:115-117. doi: 10.1016/j.ab.2016.06.014. Epub 2016 Jun 16.
本文引用的文献
1
Calibration of matrix-assisted laser desorption/ionization time-of-flight peptide mass fingerprinting spectra.
Methods Mol Biol. 2007;367:49-60. doi: 10.1385/1-59745-275-0:49.
2
Automated methods for improved protein identification by peptide mass fingerprinting.
Proteomics. 2004 Sep;4(9):2594-601. doi: 10.1002/pmic.200300804.
3
Rapid identification of proteins by peptide-mass fingerprinting.通过肽质量指纹图谱快速鉴定蛋白质。
Curr Biol. 1993 Jun 1;3(6):327-32. doi: 10.1016/0960-9822(93)90195-t.
4
Closely spaced external standard: a universal method of achieving 5 ppm mass accuracy over the entire MALDI plate in axial matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.紧密间隔外部标准：一种在轴向基质辅助激光解吸/电离飞行时间质谱中在整个MALDI板上实现5 ppm质量精度的通用方法。
Rapid Commun Mass Spectrom. 2003;17(19):2177-87. doi: 10.1002/rcm.1158.
5
Interpretation of mass spectrometry data for high-throughput proteomics.
Anal Bioanal Chem. 2003 Aug;376(7):1014-22. doi: 10.1007/s00216-003-1995-x. Epub 2003 Jul 5.
6
An improved two-step calibration method for matrix-assisted laser desorption/ionization time-of-flight mass spectra for proteomics.
Rapid Commun Mass Spectrom. 2002;16(19):1892-5. doi: 10.1002/rcm.798.
7
Use of matrix clusters and trypsin autolysis fragments as mass calibrants in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.在基质辅助激光解吸/电离飞行时间质谱中使用基质簇和胰蛋白酶自溶片段作为质量校准物。
Rapid Commun Mass Spectrom. 2002;16(18):1714-22. doi: 10.1002/rcm.775.
8
A calibration method that simplifies and improves accurate determination of peptide molecular masses by MALDI-TOF MS.
Anal Chem. 2002 Aug 1;74(15):3915-23. doi: 10.1021/ac011203o.
9
Accurate mass measurements using MALDI-TOF with delayed extraction.使用带有延迟提取的基质辅助激光解吸电离飞行时间质谱进行精确质量测量。
J Protein Chem. 1997 Jul;16(5):363-9. doi: 10.1023/a:1026376403468.
10
High-resolution matrix-assisted laser desorption/ionization in a linear time-of-flight mass spectrometer.线性飞行时间质谱仪中的高分辨率基质辅助激光解吸/电离
Anal Chem. 1995 Jul 1;67(13):1950-4. doi: 10.1021/ac00109a007.
Zotero 插件