Tendulkar Anita, Rajadhyaksha Sunil B
Department of Transfusion Medicine, Tata Memorial Hospital, Mumbai - 400 012, India.
Asian J Transfus Sci. 2009 Jul;3(2):73-7. doi: 10.4103/0973-6247.53877.
Platelet concentrate (PC) remains one of the most important support measures in thrombocytopenic patients. An efficient cell separator is a prerequisite for an optimally functioning apheresis setup. Donor blood count may undergo a temporary reduction after the procedure.
The aim was to find the extent of reduction in donor blood count (hemoglobin, hematocrit, white blood cell, and platelet) after plateletpheresis and to evaluate the cell separator for collection efficiency, processing time, and leukoreduction.
Two hundred and thirty seven procedures performed on the Amicus (N = 121), Fenwal CS-3000 Plus (N = 50) and Cobe spectra (N = 66) in a one year period were evaluated. The procedures performed on the continuous flow centrifugation (CFC) cell separators and donor blood counts (pre and post donation) done were included in the study.
The percent reduction in hemoglobin (HB), hematocrit (HCT), white blood cell (WBC) and platelet count ((PLT ct) was 2.9, 3.1, 9, 30.7 (Mean, N = 237) respectively after the procedure. The post donation PLT ct reduced to < 100x109/L (range 80-100) in five donors (N = 5/237, Amicus). The pre donation PLT ct in them was 150-200x109/L. Collection efficiency (percent) of Amicus (79.3) was better as compared to the other two machines (CS: 62.5, Cobe: 57.5). PC collected on Cobe spectra had <1x106 WBC. The donor pre donation PLT levels had a positive correlation to the product PLT yield (r = 0.30, P = 0.000).
Monitoring donor blood counts helps to avoid pheresis induced adverse events. A cautious approach is necessary in donors whose pre donation PLT ct is 150-200x109/L. The main variable in PLT yield is donor PLT ct (pre donation). High collection efficiency is a direct measure of an optimally functioning cell separator.
血小板浓缩物（PC）仍然是血小板减少症患者最重要的支持措施之一。高效的细胞分离器是单采设置正常运行的先决条件。采血过程后供体血细胞计数可能会暂时降低。
目的是确定血小板单采术后供体血细胞计数（血红蛋白、血细胞比容、白细胞和血小板）的降低程度，并评估细胞分离器的采集效率、处理时间和白细胞去除效果。
对一年内使用Amicus（N = 121）、Fenwal CS - 3000 Plus（N = 50）和Cobe spectra（N = 66）进行的237次操作进行了评估。研究纳入了在连续流动离心（CFC）细胞分离器上进行的操作以及供体献血前后的血细胞计数。
术后血红蛋白（HB）、血细胞比容（HCT）、白细胞（WBC）和血小板计数（PLT ct）的降低百分比分别为2.9%、3.1%、9%、30.7%（均值，N = 237）。5名供体（N = 5/237，Amicus）献血后的PLT ct降至<100×10⁹/L（范围80 - 100）。他们献血前的PLT ct为150 - 200×10⁹/L。与其他两台机器相比，Amicus的采集效率（百分比）更高（CS：62.5%，Cobe：57.5%）。在Cobe spectra上采集的PC白细胞<1×10⁶。供体献血前的PLT水平与产品PLT产量呈正相关（r = 0.30，P = 0.000）。
监测供体血细胞计数有助于避免单采引起的不良事件。对于献血前PLT ct为150 - 200×10⁹/L的供体，需要谨慎对待。PLT产量的主要变量是供体PLT ct（献血前）。高采集效率是细胞分离器正常运行的直接指标。
