Hans Rekha, Sharma Ratti Ram, Marwaha Neelam
Department of Transfusion Medicine, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Asian J Transfus Sci. 2019 Jan-Jun;13(1):10-16. doi: 10.4103/ajts.AJTS_86_18.
Thrombopoietin (TPO) is regulated by a feedback mechanism between megakaryocytes and platelets. This is important in plateletpheresis donors to compensate for donation-associated platelet loss.
The aim and objective of this study were to investigate changes in serum TPO levels in healthy plateletpheresis donors and its correlation with platelet recovery pattern.
Out of 50 plateletpheresis donors recruited in the study over 1 year, only 29 completed follow-up and were further analyzed. Plateletpheresis procedures were performed on two types of cell separators (TRIMA ACCEL, Terumo BCT Lakewood Colorado and AMICUS, Fresenius Kabi, Germany). Platelet parameters were estimated pre- and post-platelet donation, at 3- and 5-day postdonation. Serum TPO levels were determined using quantitative sandwich enzyme-linked immunosorbent assay technique (Raybiotech, USA) as per the protocol of the manufacturer.
The majority of donors (72%) in our study were first-time donors. The baseline platelet count was 226 ± 44 × 10/μl with a significant decline (30%; < 0.001) in postdonation phase and remained below baseline on the 3 and 5 day. The serum TPO levels increased significantly ( < 0.001) from a baseline of 227.81 (interquartile range [IQR]: 176.06) pg/ml to 269.94 (IQR: 110.68) pg/ml postdonation and remained elevated from baseline levels on the 3 and 5 day. An inverse relation was observed between change in serum TPO levels and platelet count during postdonation phase which was not statistically significant ( > 0.05).
Serum TPO levels increase significantly post plateletpheresis donation corresponding to decrease in platelet counts showing that TPO plays a vital role in compensatory mechanism after platelet loss.
血小板生成素（TPO）受巨核细胞与血小板之间反馈机制的调节。这对于血小板单采献血者补偿与献血相关的血小板损失很重要。
本研究的目的是调查健康血小板单采献血者血清TPO水平的变化及其与血小板恢复模式的相关性。
在1年的研究中招募的50名血小板单采献血者中，只有29名完成了随访并进行了进一步分析。血小板单采程序在两种类型的细胞分离器（TRIMA ACCEL，Terumo BCT公司，科罗拉多州莱克伍德市；AMICUS，费森尤斯卡比公司，德国）上进行。在血小板捐献前、捐献后以及捐献后第3天和第5天估计血小板参数。按照制造商的方案，使用定量夹心酶联免疫吸附测定技术（Raybiotech公司，美国）测定血清TPO水平。
我们研究中的大多数献血者（72%）是首次献血者。基线血小板计数为226±44×10/μl，捐献后阶段显著下降（30%；P<0.001），并在第3天和第5天仍低于基线水平。血清TPO水平从基线的227.81（四分位间距[IQR]：176.06）pg/ml显著增加（P<0.001）至捐献后的269.94（IQR：110.68）pg/ml，并在第3天和第5天保持高于基线水平。在捐献后阶段，观察到血清TPO水平变化与血小板计数之间呈负相关，但无统计学意义（P>0.05）。
血小板单采献血后血清TPO水平显著升高，与血小板计数下降相对应，表明TPO在血小板损失后的代偿机制中起重要作用。
