Suppr超能文献

在儿科人群中,血清 IgE 水平、细胞结合 IgE 和外周血细胞膜表面 IgE 受体之间的关系。

Relationships between levels of serum IgE, cell-bound IgE, and IgE-receptors on peripheral blood cells in a pediatric population.

机构信息

Division of Gastroenterology and Nutrition and EGID Centre, Children's Hospital Boston, Harvard Medical School, Boston, Massachusetts, United States of America.

出版信息

PLoS One. 2010 Aug 16;5(8):e12204. doi: 10.1371/journal.pone.0012204.

Abstract

BACKGROUND

Elevated serum immunoglobulin (Ig) E is a diagnostic marker of immediate-type allergic reactions. We hypothesize that serum IgE does not necessarily reflect total body IgE because in vivo IgE can be bound to cell surface receptors such as FcepsilonRI and FcepsilonRII (CD23). The aim of this study was to analyze the relationships between levels of serum IgE, cell-bound IgE, and IgE-receptors on peripheral blood cells in a pediatric population.

METHODOLOGY

Whole blood samples from 48 children (26 boys, 22 girls, mean age 10,3+/-5,4 years) were analyzed by flow cytometry for FcepsilonRI, CD23, and cell-bound IgE on dendritic cells (CD11c+MHC class II+), monocytes (CD14+), basophils (CD123+MHC class II-) and neutrophils (myeloperoxidase+). Total serum IgE was measured by ELISA and converted into z-units to account for age-dependent normal ranges. Correlations were calculated using Spearman rank correlation test.

PRINCIPAL FINDINGS

Dendritic cells, monocytes, basophils, and neutrophils expressed the high affinity IgE-receptor FcepsilonRI. Dendritic cells and monocytes also expressed the low affinity receptor CD23. The majority of IgE-receptor positive cells carried IgE on their surface. Expression of both IgE receptors was tightly correlated with cell-bound IgE. In general, cell-bound IgE on FcepsilonRI+ cells correlated well with serum IgE. However, some patients carried high amounts of cell-bound IgE despite low total serum IgE levels.

CONCLUSION/SIGNIFICANCE: In pediatric patients, levels of age-adjusted serum IgE, cell-bound IgE, and FcepsilonRI correlate. Even in the absence of elevated levels of serum IgE, cell-bound IgE can be detected on peripheral blood cells in a subgroup of patients.

摘要

背景

升高的血清免疫球蛋白 E(IgE)是速发型过敏反应的诊断标志物。我们假设血清 IgE 不一定反映全身 IgE,因为体内 IgE 可以与细胞表面受体如 FcepsilonRI 和 FcepsilonRII(CD23)结合。本研究旨在分析儿童人群中血清 IgE、细胞结合 IgE 和外周血细胞上 IgE 受体之间的关系。

方法

通过流式细胞术分析 48 名儿童(26 名男孩,22 名女孩,平均年龄 10.3+/-5.4 岁)的全血样本,检测树突状细胞(CD11c+MHC Ⅱ+)、单核细胞(CD14+)、嗜碱性粒细胞(CD123+MHC Ⅱ-)和中性粒细胞(髓过氧化物酶+)上的 FcepsilonRI、CD23 和细胞结合 IgE。通过 ELISA 测量总血清 IgE,并转换为 z 单位以考虑年龄相关的正常范围。使用 Spearman 秩相关检验计算相关性。

主要发现

树突状细胞、单核细胞、嗜碱性粒细胞和中性粒细胞表达高亲和力 IgE 受体 FcepsilonRI。树突状细胞和单核细胞也表达低亲和力受体 CD23。大多数 IgE 受体阳性细胞表面携带 IgE。两种 IgE 受体的表达与细胞结合 IgE 紧密相关。一般来说,FcepsilonRI+细胞上的细胞结合 IgE 与血清 IgE 相关性良好。然而,一些患者尽管血清总 IgE 水平较低,但仍存在大量细胞结合 IgE。

结论/意义:在儿科患者中,年龄调整后的血清 IgE、细胞结合 IgE 和 FcepsilonRI 水平相关。即使在血清 IgE 水平没有升高的情况下,也可以在一组患者的外周血细胞上检测到细胞结合 IgE。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fbb5/2922369/7a3fe2415ed4/pone.0012204.g001.jpg

相似文献

3
Expression of high-affinity IgE receptor on human peripheral blood dendritic cells in children.
PLoS One. 2012;7(2):e32556. doi: 10.1371/journal.pone.0032556. Epub 2012 Feb 23.
4
Age-dependent increase of IgE-binding and FcepsilonRI expression on circulating basophils in children.
Pediatr Res. 1999 Nov;46(5):603-7. doi: 10.1203/00006450-199911000-00018.
5
Serum IgE clearance is facilitated by human FcεRI internalization.
J Clin Invest. 2014 Mar;124(3):1187-98. doi: 10.1172/JCI68964. Epub 2014 Feb 24.
6
Low-affinity IgE receptor (FcepsilonRII)-mediated activation of human monocytes by both monomeric IgE and IgE/anti-IgE immune complex.
Int Immunopharmacol. 2009 Aug;9(9):1110-4. doi: 10.1016/j.intimp.2009.05.009. Epub 2009 Jun 6.
8
FcεRI expression and IgE binding by dendritic cells and basophils in allergic rhinitis and upon allergen immunotherapy.
Clin Exp Allergy. 2018 Aug;48(8):970-980. doi: 10.1111/cea.13157. Epub 2018 May 17.
9
Maternal allergy is associated with surface-bound IgE on cord blood basophils.
Pediatr Allergy Immunol. 2013 Sep;24(6):614-21. doi: 10.1111/pai.12113.

引用本文的文献

1
Atopy-related immune profiles are subject to genetic influence as evaluated using school-aged twin pairs.
Sci Adv. 2025 Jul 18;11(29):eads8033. doi: 10.1126/sciadv.ads8033. Epub 2025 Jul 16.
2
Applications of basophil activation test in paediatric allergic diseases.
World Allergy Organ J. 2024 Dec 9;17(12):100998. doi: 10.1016/j.waojou.2024.100998. eCollection 2024 Dec.
3
SHIP-1 differentially regulates IgE-induced IL-10 and antiviral responses in human monocytes.
bioRxiv. 2024 Feb 10:2024.02.07.579109. doi: 10.1101/2024.02.07.579109.
8
Novel Approaches in the Inhibition of IgE-Induced Mast Cell Reactivity in Food Allergy.
Front Immunol. 2021 Aug 12;12:613461. doi: 10.3389/fimmu.2021.613461. eCollection 2021.
9
IgE and IgG4 Repertoire in Asymptomatic HDM-Sensitized and HDM-Induced Allergic Rhinitis Patients.
Int Arch Allergy Immunol. 2021;182(12):1200-1211. doi: 10.1159/000517824. Epub 2021 Jul 28.
10
Peanut diversity and specific activity are the dominant IgE characteristics for effector cell activation in children.
J Allergy Clin Immunol. 2021 Aug;148(2):495-505.e14. doi: 10.1016/j.jaci.2021.02.029. Epub 2021 Mar 3.

本文引用的文献

1
A simple method for measuring human cell-bound IgE levels in whole blood.
J Immunol Methods. 2009 Apr 15;343(2):134-9. doi: 10.1016/j.jim.2009.01.012.
5
IgE in allergy and asthma today.
Nat Rev Immunol. 2008 Mar;8(3):205-17. doi: 10.1038/nri2273.
6
Correlation between specific immunoglobulin E levels and the severity of reactions in egg allergic patients.
Pediatr Allergy Immunol. 2008 Mar;19(2):173-9. doi: 10.1111/j.1399-3038.2007.00602.x.
8
IgE and non-IgE-mediated food allergy: treatment in 2007.
Curr Opin Allergy Clin Immunol. 2007 Jun;7(3):264-8. doi: 10.1097/ACI.0b013e32814a5607.
9
New developments in FcepsilonRI regulation, function and inhibition.
Nat Rev Immunol. 2007 May;7(5):365-78. doi: 10.1038/nri2072.
10
The low affinity IgE receptor (CD23) is cleaved by the metalloproteinase ADAM10.
J Biol Chem. 2007 May 18;282(20):14836-44. doi: 10.1074/jbc.M608414200. Epub 2007 Mar 27.

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验