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人外周血树突状细胞高亲和力 IgE 受体的表达。

Expression of high-affinity IgE receptor on human peripheral blood dendritic cells in children.

机构信息

Department of Pediatrics, Medical College of Wisconsin, Milwaukee, Wisconsin, United States of America.

出版信息

PLoS One. 2012;7(2):e32556. doi: 10.1371/journal.pone.0032556. Epub 2012 Feb 23.

DOI:10.1371/journal.pone.0032556
PMID:22384272
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3285694/
Abstract

BACKGROUND

In a mouse model of viral induced atopic disease, expression of FcεRI on dendritic cells is critical. While adult human conventional (cDC) and plasmacytoid (pDC) dendritic cells have been shown to express FcεRI, it is not known if this receptor is expressed in childhood and how its expression is governed by IgE.

METHODS

Following informed consent of subjects (n = 27, aged 12-188 months), peripheral blood was stained for surface expression of CD19, ILT7, CD1c, IgE, FcεRI and analyzed by flow cytometry (cDC: CD19(-) ILT7(-) CD1c(+); pDC: CD19(-) ILT7(+) CD1c(-)). Total and specific serum IgE levels to food and inhalant allergens were determined by ImmunoCAP, and the relationship between FcεRI expression on dendritic cells and sensitization, free IgE, cell bound IgE, and age was determined.

RESULTS

Independent of sensitization status, FcεRI expression was noted on cDC and pDC as early as 12 months of age. Serum IgE level correlated with expression of FcεRI on cDC, but not pDC. Based on the concentration of IgE, a complex relationship was found between surface bound IgE and expression of FcεRI on cDC. pDC exhibited a linear relationship of FcεRI expression and bound IgE that was consistent through all IgE concentrations.

CONCLUSIONS

In children, FcεRI expression on cDC and pDC is modulated differently by serum and cell bound IgE. IgE governance of FcεRI expression on cDC depends upon a complex relationship. Further studies are needed to determine the functional roles of FcεRI on cDC and pDC.

摘要

背景

在病毒诱导的特应性疾病的小鼠模型中，树突状细胞（DC）上 FcεRI 的表达至关重要。虽然已证实成人常规（cDC）和浆细胞样（pDC）DC 表达 FcεRI，但尚不清楚该受体是否在儿童期表达，以及 IgE 如何调控其表达。

方法

在获得受试者（n=27，年龄 12-188 个月）的知情同意后，使用流式细胞术对外周血进行表面表达 CD19、ILT7、CD1c、IgE、FcεRI 的染色，并进行分析（cDC：CD19(-)ILT7(-)CD1c(+)；pDC：CD19(-)ILT7(+)CD1c(-)）。通过 ImmunoCAP 测定食物和吸入性过敏原的总和特异性血清 IgE 水平，并确定 DC 上 FcεRI 表达与致敏、游离 IgE、细胞结合 IgE 和年龄的关系。

结果

无论致敏状态如何，早在 12 个月时，cDC 和 pDC 上就已观察到 FcεRI 的表达。血清 IgE 水平与 cDC 上 FcεRI 的表达相关，但与 pDC 无关。根据 IgE 的浓度，发现 cDC 表面结合 IgE 与 FcεRI 表达之间存在复杂的关系。pDC 上 FcεRI 表达与结合 IgE 之间呈线性关系，且在所有 IgE 浓度下均一致。

结论

在儿童中，cDC 和 pDC 上 FcεRI 的表达受血清和细胞结合 IgE 的调节方式不同。cDC 上 IgE 对 FcεRI 表达的调控依赖于复杂的关系。需要进一步的研究来确定 cDC 和 pDC 上 FcεRI 的功能作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f9f/3285694/bda43ece868f/pone.0032556.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f9f/3285694/625362788d26/pone.0032556.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f9f/3285694/24885a45d59d/pone.0032556.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f9f/3285694/bda43ece868f/pone.0032556.g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f9f/3285694/625362788d26/pone.0032556.g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f9f/3285694/24885a45d59d/pone.0032556.g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f9f/3285694/bda43ece868f/pone.0032556.g003.jpg

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