Department of Orthodontics, Tsurumi University School of Dental Medicine, Tsurumi University, 2-1-3 Tsurumi, Tsurumi-ku, Yokohama, Kanagawa 230-8501, Japan.
Histochem Cell Biol. 2010 Oct;134(4):337-43. doi: 10.1007/s00418-010-0737-3. Epub 2010 Sep 1.
Heat shock proteins (HSPs) are molecular chaperones that maintain intracellular protein homeostasis and ensure survival of cells. Continuous orthodontic force on the tooth is considered to be a type of physical stress loaded to the periodontal ligament (PDL). However, little is known about the role of HSPs during tooth movement. This study was performed to examine the expression of HSPs in the PDL during tooth movement using laser microdissection, microarray analysis, real-time RT-PCR and immunohistochemistry. Gene expression of HSPA1A in the pressure zone of the PDL was higher during 6 h of tooth movement than in the control group. Expression of HSPA1A decreased with time. HSPA1A was also detected in the pressure zone of the PDL at the protein level 24 h after the initial tissue change. These results strongly suggest that expression of HSPA1A in the PDL during early stages of tooth movement is a critical factor for tissue reaction.
热休克蛋白（HSPs）是分子伴侣，可维持细胞内蛋白质的内环境稳定，并确保细胞的存活。持续的正畸力作用于牙齿被认为是一种加载于牙周膜（PDL）的物理应激。然而，对于 HSPs 在牙齿移动过程中的作用知之甚少。本研究采用激光显微切割、微阵列分析、实时 RT-PCR 和免疫组织化学方法，研究 HSPs 在牙齿移动过程中在牙周膜中的表达。在牙齿移动 6 小时时，HSPA1A 在牙周膜的压力区的基因表达高于对照组。表达随时间而降低。在初始组织变化 24 小时后，HSPA1A 也在牙周膜的压力区被检测到。这些结果强烈表明，在牙齿移动的早期阶段，HSPA1A 在牙周膜中的表达是组织反应的关键因素。
