Descroix-Vagne M, Caillet C, Charpin G, Chikhlssa A R, Gharzouli A, Jourdan G, Desvigne A, Dumas C, Pansu D
INSERM U 45, Unité de Physiopathologie Digestive, Pavillon Hbis, Hôpital Edouard Herriot, Lyon, France.
Reprod Nutr Dev. 1990;30(6):663-72. doi: 10.1051/rnd:19900603.
The aim of this investigation was to compare the effect of an opioid, D ala2 metenkephalinamide (DAMA), on net jejunal and ileal water and electrolyte fluxes using the gut perfusion technique in the anesthetized cat. Intestinal transport was measured during intravenous infusion of serial doses of 2, 6, and 18 micrograms.kg-1.h-1 of DAMA in 6 cats. Each cat was its own control during an intravenous infusion of 150 mmol/l NaCl preceding the first dose of peptide and following the last dose of DAMA. Both jejunal and ileal segments were isolated by inflated balloons and were studied at the same time. Fifteen ml of an iso-osmolar test solution with hypo-osmolar ion contents and complementary mannitol were administered in the upstream tube and collected 1 h later in the downstream tube. In the jejunum, water secretion was dose-dependently reversed to an absorption from a control value of +0.5 +/- 0.4 to -0.83 +/- 0.5 ml.h-1.10 cm-1; in the ileum, water absorption was increased from -0.5 +/- 0.3 to -1.5 +/- 0.2 ml.h-1.10 cm-1. The net absorption of all electrolytes, ie sodium, chloride, bicarbonate, potassium and calcium also increased during peptide administration. However, a qualitative difference in the ion transport was observed between the jejunum and the ileum.
本研究的目的是在麻醉猫身上使用肠道灌注技术，比较阿片样物质D-丙氨酸2-甲硫氨酸脑啡肽酰胺（DAMA）对空肠和回肠净水分及电解质通量的影响。在6只猫中，静脉输注2、6和18微克·千克⁻¹·小时⁻¹的DAMA系列剂量时测量肠道转运。在首次给予肽之前和最后一次给予DAMA之后，每只猫在静脉输注150 mmol/l氯化钠期间作为自身对照。空肠和回肠段均通过充气球囊分离，并同时进行研究。在上游管中注入15 ml具有低渗离子含量和补充甘露醇的等渗测试溶液，1小时后在下游管中收集。在空肠中，水分分泌呈剂量依赖性逆转，从对照值+0.5±0.4变为-0.83±0.5毫升·小时⁻¹·10厘米⁻¹的吸收；在回肠中，水分吸收从-0.5±0.3增加到-1.5±0.2毫升·小时⁻¹·10厘米⁻¹。在给予肽期间，所有电解质（即钠、氯、碳酸氢盐、钾和钙）的净吸收也增加。然而，在空肠和回肠之间观察到离子转运的定性差异。
