Department of Plant Molecular Biology, University of Delhi South Campus, New Delhi, 110021, India.
Plant Mol Biol. 2010 Nov;74(4-5):395-404. doi: 10.1007/s11103-010-9682-8. Epub 2010 Sep 2.
ClpB/Hsp100 proteins act as chaperones, mediating disaggregation of denatured proteins. Recent work shows that apart from cytoplasm, these proteins are localized to nuclei, chloroplasts, mitochondria and plasma membrane. While ClpB/Hsp100 genes are essentially stress-induced (mainly heat stress) in vegetative organs of the plant body, expression of ClpB/Hsp100 proteins is noted to be constitutive in plant reproductive structures like pollen grains, developing embryos, seeds etc. With global warming looming large on the horizon, ways to genetically engineer plants against high temperature stress are urgently needed. Yeast mutants unable to synthesize active ClpB/Hsp100 protein show a clear thermosensitive phenotype. ClpB/Hsp100 proteins are implicated in high temperature stress tolerance in plants. We herein highlight the selected important facets of this protein family in plants.
ClpB/Hsp100 蛋白作为伴侣蛋白，介导变性蛋白的解聚。最近的研究表明，除了细胞质，这些蛋白还定位于细胞核、叶绿体、线粒体和质膜。虽然 ClpB/Hsp100 基因在植物体细胞的营养器官中主要受到应激（主要是热应激）诱导，但 ClpB/Hsp100 蛋白的表达在花粉粒、发育中的胚胎、种子等植物生殖结构中是组成型的。随着全球变暖迫在眉睫，迫切需要寻找基因工程植物来对抗高温胁迫的方法。不能合成活性 ClpB/Hsp100 蛋白的酵母突变体表现出明显的热敏表型。ClpB/Hsp100 蛋白参与植物对高温胁迫的耐受。本文重点介绍了该蛋白家族在植物中的一些重要方面。
