The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Lab of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, China.
Int Endod J. 2010 Dec;43(12):1116-21. doi: 10.1111/j.1365-2591.2010.01786.x. Epub 2010 Aug 31.
To investigate the cytotoxicity of bioaggregate (BA; Innovative Bioceramix, Vancouver, BC, Canada) to human periodontal ligament (PDL) fibroblasts and its effect on differentiation of human PDL fibroblasts and to compare its performance to that of mineral trioxide aggregate (MTA; Dentsply, Tulsa, OK, USA).
Cytotoxicity was assessed by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay on days 1, 2, and 3 after incubation with BA or MTA. The influence of BA and MTA on differentiation of human PDL fibroblasts on days 3, 5, and 7 was evaluated by gene expression of alkaline phosphatase (ALP) and collagen type I (COLI) via quantitative real-time polymerase chain reaction (PCR). The data were analysed by one-way analysis of variance followed by Tukey's test.
Cell numbers in the BA group were similar to that of the control group throughout the culture period, whereas MTA suppressed the proliferation of fibroblasts. ALP expression was significantly increased in the BA group on day 7, whilst it was enhanced by MTA on day 3. Gene expression of COLI was induced by both BA and MTA compared to the control group.
Bioaggregate was nontoxic to human PDL fibroblasts and appeared to induce the differentiation of human PDL fibroblasts.
研究生物骨料（BA；加拿大温哥华创新生物陶瓷公司）对人牙周膜（PDL）成纤维细胞的细胞毒性及其对人 PDL 成纤维细胞分化的影响，并将其性能与三氧化矿物凝聚体（MTA；美国俄克拉荷马州塔尔萨登士柏公司）进行比较。
用 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）法在孵育 BA 或 MTA 后第 1、2 和 3 天评估细胞毒性。通过定量实时聚合酶链反应（PCR）检测碱性磷酸酶（ALP）和 I 型胶原（COLI）的基因表达，评估 BA 和 MTA 对人 PDL 成纤维细胞分化的影响。数据采用单因素方差分析，然后进行 Tukey 检验。
在整个培养期间，BA 组的细胞数量与对照组相似，而 MTA 抑制了成纤维细胞的增殖。BA 组在第 7 天的 ALP 表达明显增加，而 MTA 在第 3 天增强了其表达。与对照组相比，BA 和 MTA 均诱导 COLI 基因的表达。
生物骨料对人牙周膜成纤维细胞无毒性，并能诱导人牙周膜成纤维细胞的分化。
