Department of Ophthalmology, University of Rostock, D-18055 Rostock, Germany.
Br J Ophthalmol. 2010 Sep;94(9):1133-5. doi: 10.1136/bjo.2009.175489.
To produce two-dimensional reconstruction maps of the subepithelial nerve plexus (SEP) in living cornea by in vivo laser scanning confocal microscopy in real time.
In vivo confocal laser scanning microscopy (Heidelberg Retinal Tomograph II in conjunction with the Rostock Cornea Module) was performed on normal eyes (n=6) and eyes after laser-assisted in situ keratomileusis (LASIK) (n=4). Source data (frame rate 30 Hz) were used to create large-scale maps of the scanned area in Automatic Real Time composite mode. The algorithm aligns single live images onto the previously mapped composite image using landmark feature-based image processing.
Real-time mapping of the SEP was performed on a large-scale area up to 3.2x3.2 mm (3072x3072 pixels) in healthy subjects and in post-LASIK patients. Two-dimensional structures of the SEP were imaged in all 10 eyes. Mapping quality as well as acquisition time were dependent on subject compliance and examiner experience.
The described method permits real-time in vivo mapping of the SEP, thus providing the necessary basis for statistically robust conclusions concerning morphometric plexus alterations.
通过活体激光共聚焦显微镜实时产生活体角膜上皮下神经丛（SEP）的二维重建图。
对正常眼（n=6）和激光辅助原位角膜磨镶术（LASIK）后眼（n=4）进行活体共焦激光扫描显微镜检查（Heidelberg Retinal Tomograph II 联合 Rostock Cornea Module）。使用源数据（帧率 30 Hz）在自动实时复合模式下创建扫描区域的大比例尺地图。该算法使用基于特征点的图像处理将单个实时图像与之前映射的复合图像对齐。
在健康受试者和 LASIK 后患者中，对 3.2x3.2mm（3072x3072 像素）的大面积实时映射 SEP。在所有 10 只眼中都可以成像 SEP 的二维结构。映射质量和采集时间取决于受试者的依从性和检查者的经验。
所描述的方法允许实时体内 SEP 映射，从而为有关形态丛改变的统计学稳健结论提供必要的基础。
