通过共聚焦激光扫描显微镜对角膜进行细胞三维成像。
Cellular 3D imaging of the cornea by confocal laser scanning microscopy.
作者信息
Bohn Sebastian, Sperlich Karsten, Allgeier Stephan, Bartschat Andreas, Prakasam Ruby, Reichert Klaus-Martin, Stolz Heinrich, Guthoff Rudolf, Mikut Ralf, Köhler Bernd, Stachs Oliver
机构信息
Department of Ophthalmology, University Medical Center Rostock, 18057 Rostock, Germany.
Institute for Automation and Applied Informatics, Karlsruhe Institute of Technology, 76131 Karlsruhe, Germany.
出版信息
Biomed Opt Express. 2018 May 1;9(6):2511-2525. doi: 10.1364/BOE.9.002511. eCollection 2018 Jun 1.
Abstract
We present an confocal laser scanning microscopy based method for large 3D reconstruction of the cornea on a cellular level with cropped volume sizes up to 266 x 286 x 396 µm. The microscope objective used is equipped with a piezo actuator for automated, fast and precise closed-loop focal plane control. Furthermore, we present a novel concave surface contact cap, which significantly reduces eye movements by up to 87%, hence increasing the overlapping image area of the whole stack. This increases the cuboid volume of the generated 3D reconstruction significantly. The possibility to generate oblique sections using isotropic volume stacks opens the window to slit lamp microscopy on a cellular level.
摘要
我们提出了一种基于共聚焦激光扫描显微镜的方法，用于在细胞水平上对角膜进行大尺寸三维重建，裁剪后的体积尺寸可达266×286×396µm。所使用的显微镜物镜配备了一个压电致动器，用于自动、快速且精确的闭环焦平面控制。此外，我们还展示了一种新型的凹面接触帽，它能将眼球运动显著减少多达87%，从而增加整个堆栈的重叠图像区域。这显著增加了所生成三维重建的长方体体积。利用各向同性体积堆栈生成斜截面的可能性，为细胞水平的裂隙灯显微镜检查打开了一扇窗。
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