Department of Botany, St. John's College, Agra, 282002, India.
Environ Monit Assess. 2011 Jul;178(1-4):373-81. doi: 10.1007/s10661-010-1696-9. Epub 2010 Sep 3.
Toxicity of lead in soil is well documented and established. Phytoremediation has gained attention as a cheap, easily applicable, and eco-friendly clean-up technology. Chemical methods are used to assess exact levels and type of pollutants but heavy metal content in soil can also be evaluated indirectly by estimation of phytotoxicity levels using bioassays. Plant bioassays through fast germinating cereals can indicate not only the level of pollution and its effects on growth and survival but also the progress of phytoremediation process. The performance of barley Hordeum vulgare L. seedlings as bioassay for assessment of changes in the levels of lead (Pb) at three concentrations, i.e., 300 (T(1)), 600 (T(2)), and 1,200 ppm (T(3)) in the soil was evaluated while testing the efficiency of Crinum asiaticum L. as a phytoremedial tool. At the first assessment, i.e., 30 DAT (days after treatment) shoot and root lengths of seedlings decreased with increasing concentrations of Pb. As the study progressed, a decrease in levels of Pb was accompanied by better germinability and growth of barley. At 120 DAT seedling growth in all the treatments were comparable to control. In T(1), T(2), and T(3) soils, 74.5%, 83.7%, and 91.2% reduction in lead content was observed at 120 DAT. Highly significant correlations between decreasing pollutant (Pb) content in the soil, seed germination, and seedling growth of barley H. vulgare were found. The differences in root and shoot length as well as overall growth pattern are indicative of the suitability of barley as a bio-monitoring tool.
土壤中铅的毒性已有充分的文献记载和证明。植物修复作为一种廉价、易于应用且环保的清洁技术，已受到关注。化学方法用于评估污染物的确切水平和类型，但也可以通过生物测定法估算植物毒性水平间接评估土壤中的重金属含量。利用快速发芽的谷类植物进行植物生物测定不仅可以指示污染水平及其对生长和存活的影响，还可以指示植物修复过程的进展。大麦（Hordeum vulgare L.）幼苗作为生物测定法，评估土壤中铅（Pb）三种浓度（即 300（T(1)）、600（T(2)）和 1200 ppm（T(3)））水平变化的性能，同时测试百合（Crinum asiaticum L.）作为一种植物修复工具的效率。在第一次评估时，即处理后 30 天（DAT），幼苗的茎和根长随着 Pb 浓度的增加而降低。随着研究的进展，Pb 水平的降低伴随着大麦更好的发芽率和生长。在 120 DAT，所有处理的幼苗生长都与对照相当。在 T(1)、T(2)和 T(3)土壤中，在 120 DAT 时观察到 Pb 含量分别减少了 74.5%、83.7%和 91.2%。在土壤中减少污染物（Pb）含量、大麦种子发芽和幼苗生长之间发现了高度显著的相关性。根长和茎长以及整体生长模式的差异表明大麦作为生物监测工具的适用性。
