Sun Guoling, Qian Dawei, Duan Jin'ao, Li Xiangming, Wan Jianyi, Guo Jianming
School of Pharmacological Science, Jiangsu University, Zhenjiang 212013, China.
Zhongguo Zhong Yao Za Zhi. 2010 Jun;35(12):1580-5. doi: 10.4268/cjcmm20101217.
To analyze naringin, naringenin and its metabolites in rat plasma after intragastric administration of exocarpium Citri grandis alcohol extract.
Rat blood samples were collected 1.0 hour after oral administration of 50 g x kg(-1) exocarpium Citri grandis alcohol extract and analyzed by UPLC-Q-TOF with MS(E) function. The post-acquisition data were processed using Metabolynx.
Naringin (M1), naringenin (M2), naringin-5-O-glucuronide (M3), naringin-4-O-glucuronide (M4), glucuronide conjugate of naringenin (M5), naringin-4-O-sulfate (M6), methylated conjugate of hydroxylated naringenin (M7), glucuronide and sulfate conjugate of naringenin (M8), glucuronide conjugate of hydroxylated naringenin (M9) in rat plasma were detected. M3, M4, M6 were first reported as the metabolites of naringin. M7, M9 were first reported as the metabolites of naringenin.
The results indicated that naringin, naringenin can be metabolited as the forms of glucuronidation, sulfation and naringenin can also be metabolited as the forms of methylation with hydroxylation and glucuronidation with hydroxylation in vivo after administration.
分析大鼠灌胃给予化橘红醇提取物后血浆中的柚皮苷、柚皮素及其代谢产物。
大鼠口服50 g·kg⁻¹化橘红醇提取物1.0小时后采集血样，采用具有MS(E)功能的超高效液相色谱-四极杆飞行时间质谱联用仪进行分析。采集后的数据使用Metabolynx进行处理。
在大鼠血浆中检测到柚皮苷（M1）、柚皮素（M2）、柚皮苷-5-O-葡萄糖醛酸苷（M3）、柚皮苷-4-O-葡萄糖醛酸苷（M4）、柚皮素葡萄糖醛酸结合物（M5）、柚皮苷-4-O-硫酸盐（M6）、羟基化柚皮素甲基化结合物（M7）、柚皮素葡萄糖醛酸和硫酸盐结合物（M8）、羟基化柚皮素葡萄糖醛酸结合物（M9）。M3、M4、M6首次被报道为柚皮苷的代谢产物。M7、M9首次被报道为柚皮素的代谢产物。
结果表明，柚皮苷、柚皮素在给药后体内可通过葡萄糖醛酸化、硫酸化形式代谢，柚皮素还可通过羟基化甲基化、羟基化葡萄糖醛酸化形式代谢。
