Grupo de Bioprocesos y Bioprospección, Instituto de Biotecnología, Universidad Nacional de Colombia, Sede Bogotá, Bogotá, D.C., 111321, Colombia.
Laboratorio de Biotecnología Aplicada, Grupo de Biotecnología Ambiental e Industrial (GBAI), Departamento de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana, 110231, Bogotá, D.C., Colombia.
J Microbiol. 2017 Sep;55(9):711-719. doi: 10.1007/s12275-017-6282-1. Epub 2017 Sep 2.
The objective of this study was to evaluate the potential of eight fungal isolates obtained from soils in rice crops for straw degradation in situ. From the initial eight isolates, Pleurotus ostreatus T1.1 and Penicillium sp. HC1 were selected for further characterization based on qualitative cellulolytic enzyme production and capacity to use rice straw as a sole carbon source. Subsequently, cellulolytic, xylanolytic, and lignolytic (Pleurotus ostreatus) activity on carboxymethyl cellulose, oat xylan, and rice straw with different nitrogen sources was evaluated. From the results obtained it was concluded both isolates are capable to produce enzymes necessary for rice straw degradation. However, their production is dependent upon carbon and nitrogen source. Last, it was established that Pleurotus ostreatus T1.1 and Penicillium sp. HC1 capability to colonize and mineralize rice straw, in mono-and co-culture, without affecting nitrogen soil content.
本研究旨在评估从水稻土壤中获得的 8 株真菌分离物在原位降解秸秆的潜力。从最初的 8 个分离物中，根据定性纤维素酶产生和利用水稻秸秆作为唯一碳源的能力，选择了糙皮侧耳 T1.1 和青霉 HC1 进行进一步的特性描述。随后，评估了纤维素酶、木聚糖酶和木质素酶（糙皮侧耳）在羧甲基纤维素、燕麦木聚糖和不同氮源的水稻秸秆上的活性。从获得的结果得出结论，两种分离物都能够产生降解水稻秸秆所需的酶。然而，它们的产生取决于碳源和氮源。最后，确定糙皮侧耳 T1.1 和青霉 HC1 能够在单培养和共培养中定植和矿化水稻秸秆，而不影响土壤中的氮含量。
