Onodera T, Jenson A B, Yoon J W, Notkins A L
Science. 1978 Aug 11;201(4355):529-31. doi: 10.1126/science.208156.
Reovirus type 3, passaged in pancreatic beta-cell cultures, produced an insulitis when inoculated into 1- to 2-week-old mice. By means of a double-label antibody technique, in which we used fluorescein-labeled antibody to reovirus and rhodamine-labeled antibody to insulin, reovirus antigens were found in beta cells. By electron microscopy, viral particles in different stages of morphogenesis were observed in insulin-containing beta cells but not glucagon-containing alpha cells. The infection resulted in destruction of beta cells, reduction in the insulin content of the pancreas, and alteration in the host's capacity to respond normally to a glucose tolerance test.
3型呼肠孤病毒在胰腺β细胞培养物中传代后，接种到1至2周龄小鼠体内会引发胰岛炎。通过双标记抗体技术，即使用荧光素标记的呼肠孤病毒抗体和罗丹明标记的胰岛素抗体，在β细胞中发现了呼肠孤病毒抗原。通过电子显微镜观察，在含胰岛素的β细胞中发现了处于不同形态发生阶段的病毒颗粒，而在含胰高血糖素的α细胞中未发现。这种感染导致β细胞破坏、胰腺胰岛素含量降低以及宿主对葡萄糖耐量试验正常反应能力的改变。
