Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai, PR China.
Pharm Biol. 2011 Jan;49(1):86-93. doi: 10.3109/13880209.2010.499517. Epub 2010 Sep 3.
Hypertrophic scarring following surgical procedures, trauma and especially burns can lead to severe functional and cosmetic impairment, causing a decrease in the quality of life. Although a wide choice of treatments is offered, few therapeutic methods are universally accepted because of their side effects.
The effects of the essential oil (EO) extracted from rhizomes of Ligusticum chuanxiong Hort. (Umbelliferae) in human hypertrophic scar fibroblasts (HSFs) are investigated for the first time.
Chemical composition of hydrodistilled EO obtained from rhizomes of Ligusticum chuanxiong was analyzed by gas chromatography-mass spectrometry (GC-MS). The effects of EO on cell viability, apoptosis rate, mitochondrial membrane potential (MMP), lactate dehydrogenase (LDH), reactive oxygen species (ROS) and caspase-3 in HSFs were investigated.
The experimental results showed that EO significantly inhibited cell viability, elicited morphological changes and induced apoptosis in HSFs. EO also evidently increased the loss of MMP, the levels of LDH release and cellular ROS production, and the activity of caspase-3.
EO-induced apoptosis was at least partially carried out via destruction of the intracellular antioxidant system and elicitation of excessive ROS accumulation in HSFs, which impaired mitochondrial membranes and elicited caspase-3 activation. EO could be an effective cure for human hypertrophic scar.
手术、创伤，尤其是烧伤后的增生性瘢痕会导致严重的功能和美容损伤，降低生活质量。尽管提供了广泛的治疗选择，但由于其副作用，很少有治疗方法被普遍接受。
首次研究了来自川芎（伞形科）根茎的精油（EO）对人增生性瘢痕成纤维细胞（HSFs）的影响。
通过气相色谱-质谱联用（GC-MS）分析水蒸馏得到的川芎根茎 EO 的化学成分。研究了 EO 对 HSFs 细胞活力、凋亡率、线粒体膜电位（MMP）、乳酸脱氢酶（LDH）、活性氧（ROS）和半胱天冬酶-3 的影响。
实验结果表明，EO 显著抑制 HSFs 的细胞活力，引起形态变化并诱导其凋亡。EO 还明显增加 MMP 的丧失、LDH 释放水平和细胞内 ROS 的产生，以及半胱天冬酶-3 的活性。
EO 诱导的细胞凋亡至少部分是通过破坏细胞内抗氧化系统和引发 HSFs 中过量 ROS 积累来实现的，这破坏了线粒体膜并引发了半胱天冬酶-3 的激活。EO 可能是治疗人增生性瘢痕的有效方法。
