二维连续介质中具有非零半径的扩散粒子的渗流阈。

Two-dimensional continuum percolation threshold for diffusing particles of nonzero radius.

机构信息

Department of Biochemistry and Molecular Medicine, University of California, Davis, California, USA.

出版信息

Biophys J. 2010 Sep 8;99(5):1490-9. doi: 10.1016/j.bpj.2010.06.033.

Abstract

Lateral diffusion in the plasma membrane is obstructed by proteins bound to the cytoskeleton. The most important parameter describing obstructed diffusion is the percolation threshold. The thresholds are well known for point tracers, but for tracers of nonzero radius, the threshold depends on the excluded area, not just the obstacle concentration. Here thresholds are obtained for circular obstacles on the continuum. Random obstacle configurations are generated by Brownian dynamics or Monte Carlo methods, the obstacles are immobilized, and the percolation threshold is obtained by solving a bond percolation problem on the Voronoi diagram of the obstacles. The percolation threshold is expressed as the diameter of the largest tracer that can cross a set of immobile obstacles at a prescribed number density. For random overlapping obstacles, the results agree with the known analytical solution quantitatively. When the obstacles are soft disks with a 1/r(12) repulsion, the percolating diameter is approximately 20% lower than for overlapping obstacles. A percolation model predicts that the threshold is highly sensitive to the tracer radius. To our knowledge, such a strong dependence has so far not been reported for the plasma membrane, suggesting that percolation is not the factor controlling lateral diffusion. A definitive experiment is proposed.

摘要

质膜中的侧向扩散会受到与细胞骨架结合的蛋白质的阻碍。描述阻碍扩散最重要的参数是渗透阈值。对于点示踪剂,这些阈值是已知的,但对于非零半径的示踪剂,阈值不仅取决于障碍物浓度,还取决于被排除的面积。本文在连续介质中获得了圆形障碍物的阈值。通过布朗动力学或蒙特卡罗方法生成随机障碍物配置,将障碍物固定,并通过在障碍物的 Voronoi 图上求解键渗滤问题来获得渗滤阈值。渗滤阈值表示可以以规定的密度穿过一组固定障碍物的最大示踪剂的直径。对于随机重叠障碍物,结果与已知的解析解在数量上是一致的。当障碍物为具有 1/r(12)排斥力的软磁盘时,渗透直径比重叠障碍物低约 20%。渗滤模型预测,阈值对示踪剂半径非常敏感。据我们所知,到目前为止,这种强依赖性尚未在质膜中报道过,这表明渗滤不是控制侧向扩散的因素。提出了一个明确的实验。

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