State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou, China.
J Genet Genomics. 2010 Aug;37(8):523-31. doi: 10.1016/S1673-8527(09)60072-2.
A residual heterozygous line (RHL) carrying a heterozygous segment between two SSR loci RM11 and RM134 on the rice chromosome 7 was selected from a set of recombinant inbred lines from the cross D50 (javanica)/HB277 (indica). The former parent produces much longer grains than the latter. Selfed progenies of this selection were analyzed genotypically (SSRs) and phenotypically (grain length). Grain length was discontinuously variable in the mapping populations, allowing for the placement of this QTL qGL7-2 within a approximately 4.8 cM interval defined by RM351 and RM234. A set of new markers within this region were developed, which narrowed the QTL to a 278 kb region defined by the markers Indel1 and RM21945. This region contains 49 predicted genes. The results also suggest that the novel allele for grain length will be used for the application of marker assisted selection for the improvement of grain length.
从 D50（爪哇稻）/HB277（籼稻）杂交重组自交系群体中，选择了一个在水稻第 7 号染色体上两个 SSR 标记 RM11 和 RM134 之间存在杂合片段的残余杂合系（RHL）。前者的粒长明显长于后者。该选择的自交后代在基因型（SSR）和表型（粒长）上进行了分析。在作图群体中，粒长呈不连续变异，使得该 QTL qGL7-2 定位于由 RM351 和 RM234 定义的约 4.8cM 区间内。在该区域内开发了一组新的标记，将 QTL 缩小到由标记 Indel1 和 RM21945 定义的 278kb 区域。该区域包含 49 个预测基因。结果还表明，粒长的新等位基因将用于标记辅助选择改良粒长的应用。
