Aslam Kashif, Naveed Shahzad Amir, Sabar Muhammad, Shabir Ghulam, Shah Shahid Masood, Khan Abdul Rehman, Shah Muhammad Musaddiq, Fiaz Sajid, Xu Jianlong, Arif Muhammad
National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Pakistan.
Institute of Molecular Biology and Biotechnology, BZ University, Multan, Pakistan.
Front Genet. 2022 Aug 19;13:955347. doi: 10.3389/fgene.2022.955347. eCollection 2022.
Grain appearance is one of the most important attributes of rice. It is determined by grain size, shape, and weight, which in turn influences the rice yield and market value. In this study, QTLs for grain length, grain width, grain length/width ratio, and grain weight were mapped using the high-throughput indica/indica SNP platforms. The population of the mega indica variety IR64 and the high-quality aromatic variety Sadri from Iran was phenotyped. Based on this phenotypic data, plants of 94 F families including both parents were selected. A linkage map analysis of 210 SNP markers identified 14 QTLs controlling the grain length, grain width, length/width ratio, and 1,000 grain weight. Among these 14, one important region containing the QTLs for all the four studies' traits was mapped on chromosome 8. It was derived from Sadri for the decreased length/width ratio and increased grain weight. This study demonstrated the speed and efficiency in using multiplex SNP genotyping for QTL analysis. Moreover, this study identified four novel QTLs (qGL8, qTGW8, qLWR8, and qGW8) sharing the same position on chromosome 8 which were linked with grain quality characteristics between one indica and one aromatic variety. It will enable more precise marker-assisted selection for grain weight, shape, and size. Further in-depth studies are required to dissect this region of interest and identify the related gene(s).
粒形是水稻最重要的特性之一。它由粒长、粒宽和粒重决定，而这些反过来又会影响水稻产量和市场价值。在本研究中，利用高通量籼稻/籼稻单核苷酸多态性（SNP）平台对控制粒长、粒宽、长宽比和粒重的数量性状基因座（QTL）进行了定位。对超级籼稻品种IR64和来自伊朗的优质香稻品种萨德里的群体进行了表型分析。基于这些表型数据，从包括双亲在内的94个F家系中选择了植株。对210个SNP标记进行连锁图谱分析，鉴定出14个控制粒长、粒宽、长宽比和千粒重的QTL。在这14个QTL中，有一个重要区域包含了所有四项研究性状的QTL，该区域位于第8号染色体上。它来自萨德里，具有降低的长宽比和增加的粒重。本研究证明了使用多重SNP基因分型进行QTL分析的速度和效率。此外，本研究还鉴定出四个新的QTL（qGL8、qTGW8、qLWR8和qGW8），它们在第8号染色体上具有相同位置，与一个籼稻品种和一个香稻品种之间的粒质特性相关。这将使对粒重、形状和大小的更精确的标记辅助选择成为可能。需要进一步深入研究来剖析这个感兴趣的区域并鉴定相关基因。
