通过稳定同位素辅助核磁共振光谱法探究糖缀合物的动力学和相互作用。
Dynamics and interactions of glycoconjugates probed by stable-isotope-assisted NMR spectroscopy.
作者信息
Yamaguchi Yoshiki, Kato Koichi
机构信息
Systems Glycobiology Research Group, Chemical Biology Department, RIKEN, Advanced Science Institute, Hirosawa, Wako, Japan.
出版信息
Methods Enzymol. 2010;478:305-22. doi: 10.1016/S0076-6879(10)78015-0.
Unique advantages offered by nuclear magnetic resonance (NMR) spectroscopy provide high-resolution information not only on structures but also on dynamics and interactions of glycoconjugates in solution. These benefits are further enhanced by applying stable-isotope-labeling techniques, which we have developed. Our stable-isotope-assisted NMR analyses of immunoglobulin G-Fc glycoproteins and the glycopeptides derived therefrom are here presented in terms of the dynamics and interactions of glycoconjugates.
核磁共振（NMR）光谱所具有的独特优势不仅能提供关于糖缀合物在溶液中的结构、动力学和相互作用的高分辨率信息。通过应用我们所开发的稳定同位素标记技术，这些优势得到了进一步增强。我们在此展示了利用稳定同位素辅助的NMR分析免疫球蛋白G-Fc糖蛋白及其衍生糖肽所获得的关于糖缀合物的动力学和相互作用的结果。
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