监测人免疫球蛋白 G 与甲基乙二醛和乙二醛的非酶糖化：光谱研究。
Monitoring nonenzymatic glycation of human immunoglobulin G by methylglyoxal and glyoxal: A spectroscopic study.
机构信息
Department of Chemistry, University of Rhode Island, Kingston, 02881, USA.
出版信息
Anal Biochem. 2011 Jan 1;408(1):59-63. doi: 10.1016/j.ab.2010.08.038. Epub 2010 Sep 17.
Abstract
The accumulation of dicarbonyl compounds, methylglyoxal (MG) and glyoxal (G), has been observed in diabetic conditions. They are formed from nonoxidative mechanisms in anaerobic glycolysis and lipid peroxidation, and they act as advanced glycation endproduct (AGE) precursors. The objective of this study was to monitor and characterize the AGE formation of human immunoglobulin G (hIgG) by MG and G using ultraviolet (UV) and fluorescence spectroscopy, circular dichroism (CD), and matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS). hIgG was incubated over time with MG and G at different concentrations. Formation of AGE was monitored by UV and fluorescence spectroscopy. The effect of AGE formation on secondary structure of hIgG was studied by CD. Comparison of AGE profile for MG and G was performed by MALDI-MS. Both MG and G formed AGE, with MG being nearly twice as reactive as G. The combination of these techniques is a convenient method for evaluating and characterizing the AGE proteins.
摘要
在糖尿病患者中观察到二羰基化合物（MG 和 G）的积累。它们是在无氧糖酵解和脂质过氧化的非氧化机制中形成的，并且它们作为晚期糖基化终产物（AGE）前体起作用。本研究的目的是使用紫外（UV）和荧光光谱法、圆二色性（CD）和基质辅助激光解吸/电离 - 质谱法（ MALDI-MS）监测和表征 MG 和 G 对人免疫球蛋白 G（hIgG）的 AGE 形成。hIgG 在不同浓度的 MG 和 G 中随时间孵育。通过 UV 和荧光光谱法监测 AGE 的形成。通过 CD 研究 AGE 形成对 hIgG 二级结构的影响。通过 MALDI-MS 对 MG 和 G 的 AGE 谱进行比较。MG 和 G 均形成 AGE，MG 的反应性几乎是 G 的两倍。这些技术的结合是评估和表征 AGE 蛋白的一种便捷方法。
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