Department of Agronomy, College of Agriculture and Biotechnology, Huajiachi Campus, Zhejiang University, Hangzhou 310029, China.
Department of Agronomy, College of Agriculture and Biotechnology, Huajiachi Campus, Zhejiang University, Hangzhou 310029, China.
J Hazard Mater. 2010 Dec 15;184(1-3):191-203. doi: 10.1016/j.jhazmat.2010.08.023. Epub 2010 Aug 14.
The present study investigated Typha angustifolia leaf proteome in response to Cr, Cd and Pb stress. T. angustifolia of 90 (D90) and 130d (D130) old plants were subjected to 1mM Cr, Cd and Pb and samples were collected 30d after treatment. 2-DE coupled with MS (mass spectrometry) was used to analyze and identify Cr, Cd and Pb-responsive proteins. More than 1600 protein spots were reproducibly detected on each gel, wherein 44, 46, 66 and 33, 26, 62 spots in D90 and D130 samples were differentially expressed by Cr, Cd, Pb over the control, respectively. Of these differentially expressed proteins, 3, 1, 8 overlapped in D90 and D130; while 5, 8, 5 with regulation factors above 3 in one of D90 or D130 samples. Total of 22 and 4 up- and down-regulated proteins were identified using MS and data bank analysis. Cr-induced expression of ATP synthase, RuBisCO small subunit and coproporphyrinogen III oxidase; Cd-induced RuBisCO large subunit; Pb up-regulated carbohydrate metabolic pathway enzymes of fructokinase, and improved RuBisCO activase and large subunit, Mg-protoporphyrin IX chelatase. Contrarily, elF4F was inhibited by Cr/Pb, chloroplast FtsZ-like protein and GF14 omega impeded by Cd and Pb, respectively.
本研究调查了镉、铬和铅胁迫下狭叶香蒲叶片蛋白质组的变化。选用 90d（D90）和 130d（D130）龄的狭叶香蒲植株，用 1mM 的 Cd、Cr 和 Pb 处理 30d 后收集样品。采用 2-DE 结合 MS（质谱）分析和鉴定 Cr、Cd 和 Pb 响应蛋白。每张凝胶上可重复性地检测到 1600 多个蛋白点，其中 44、46、66 和 33、26、62 个蛋白点在 D90 和 D130 样品中分别对 Cr、Cd、Pb 表现出比对照更高的表达水平。这些差异表达蛋白中，有 3、1、8 个蛋白在 D90 和 D130 中重叠；有 5、8、5 个蛋白在 D90 或 D130 中有一种处理的调控因子超过 3。通过 MS 和数据库分析共鉴定出 22 个上调和 4 个下调蛋白。Cr 诱导 ATP 合酶、RuBisCO 小亚基和原卟啉原氧化酶表达；Cd 诱导 RuBisCO 大亚基；Pb 上调果糖激酶等糖代谢途径的酶，提高 RuBisCO 激活酶和大亚基，Mg-原卟啉 IX 螯合酶。相反，Cr/Pb 抑制 eIF4F，Cd 和 Pb 分别抑制质体 FtsZ 样蛋白和 GF14 omega。
