Department of Pharmacy, University of Peshawar, Peshawar, Pakistan.
Pharm Biol. 2010 Oct;48(10):1115-8. doi: 10.3109/13880200903517950.
Eremostachys laciniata (L.) Bunge (Lamiaceae), which has been reported as a rich source of flavonoids, is one of the rarely explored species of the genus Eremostachys.
In this study, the crude methanol extract and different fractions of E. laciniata were investigated for in vivo anti-inflammatory properties.
Shade-dried leaves of E. laciniata were exhaustively extracted by percolation with methanol (80%) to obtain 250 g of crude methanol extract (El), followed by fractionation with different organic solvents to get the n-hexane (Elh), chloroform (Elc), ethyl acetate (Ele), butanol (Elb), and water (Elw) fractions. An in vivo anti-inflammatory study of the crude extract and sub-crude fractions was carried out in rats using the carrageenan model.
The Ele fraction was found to be the most potent inhibitor of edema formation by inducing a maximum inhibitory effect of 74.2% at the 300 mg/kg dose, during 3 h post carrageenan injection. The El extract and Elc fraction also showed good anti-inflammatory properties at the same dose.
The demonstration of excellent anti-inflammatory activity by the plant chiefly concentrating in the Ele fraction and the appearance of peak activity in the latter phase of the experiment suggested the presence of relatively low-polar substances with arachidonic acid metabolite inhibition property.
The plant may be an excellent source in the future for activity-guided isolation of important anti-inflammatory substances.
木立芦竹（L.）Bunge（唇形科），据报道富含黄酮类化合物，是木立芦竹属中研究较少的物种之一。
本研究考察了木立芦竹的粗甲醇提取物和不同部位的体内抗炎特性。
将木立芦竹的阴干叶片用甲醇（80%）渗漉提取，得到 250 g 粗甲醇提取物（El），然后用不同有机溶剂进行分级，得到正己烷（Elh）、氯仿（Elc）、乙酸乙酯（Ele）、正丁醇（Elb）和水（Elw）部分。在大鼠中使用角叉菜胶模型对粗提取物和亚粗提部分进行体内抗炎研究。
Ele 部分被发现是最有效的水肿形成抑制剂，在角叉菜胶注射后 3 h 时，在 300 mg/kg 剂量下诱导最大抑制作用 74.2%。El 提取物和 Elc 部分在相同剂量下也表现出良好的抗炎作用。
植物表现出优异的抗炎活性，主要集中在 Ele 部分，并且在实验的后期阶段出现活性峰值，这表明存在具有花生四烯酸代谢物抑制特性的相对低极性物质。
该植物将来可能是活性导向分离重要抗炎物质的极好来源。
