Asnaashari Solmaz, Delazar Abbas, Asgharian Parina, Lotfipour Farzaneh, Bamdad Moghaddam Sedigeh, Heshmati Afshar Fariba
Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Iran J Pharm Res. 2017 Winter;16(1):306-314.
The current study evaluated the general toxicity, antioxidant, antimicrobial, and cytotoxic activity of extracts obtained from the rhizomes of (Labiatae) as well as analyzed the potent extracts using GC-MS. Extracts of in n-hexane, dichloromethane (DCM) and methanol (MeOH) were prepared using a Soxhlet apparatus. The antioxidant activity of the extracts was evaluated for free radical scavenging activity by DPPH assay. The antimicrobial activity of samples was determined by disc diffusion and brine shrimp lethality assay (BSLA) was used to assess general toxicity. The cytotoxicity of each extract was determined by MTT assay against human colorectal adenocarcinoma (HT29), human lung carcinoma (A549) and a normal cell line (human umbilical vein endothelial cells, HUVEC). The MeOH extract showed significant antioxidant activity and the n-hexane and DCM extracts showed promising activity against gram-positive species when compared with amikacin as a standard. Moreover, the n-hexane extract displayed the most potent activity in general toxicity assay. The results showed that all three extracts have cytotoxic effects against the A549 cell line. In the case of HT29 cell lines, only the DCM extract exhibited cytotoxicity. Interestingly, none of the extracts showed significant cytotoxic activity against the HUVEC cell line. The bioassay-guided identification of constituents showed the presence of fatty acids and steroids as the compounds responsible for bioactivity in the non-polar extracts.
本研究评估了唇形科植物根茎提取物的一般毒性、抗氧化、抗菌和细胞毒性活性,并使用气相色谱-质谱联用仪(GC-MS)分析了有效提取物。使用索氏提取器制备了该植物在正己烷、二氯甲烷(DCM)和甲醇(MeOH)中的提取物。通过DPPH法评估提取物的抗氧化活性,以检测其自由基清除能力。通过纸片扩散法测定样品的抗菌活性,并使用卤虫致死率试验(BSLA)评估一般毒性。通过MTT法测定每种提取物对人结肠腺癌(HT29)、人肺癌(A549)和正常细胞系(人脐静脉内皮细胞,HUVEC)的细胞毒性。与作为标准的阿米卡星相比,甲醇提取物显示出显著的抗氧化活性,正己烷和二氯甲烷提取物对革兰氏阳性菌表现出有前景的活性。此外,正己烷提取物在一般毒性试验中表现出最强的活性。结果表明,所有三种提取物对A549细胞系均有细胞毒性作用。在HT29细胞系中,只有二氯甲烷提取物表现出细胞毒性。有趣的是,没有一种提取物对HUVEC细胞系表现出显著的细胞毒性活性。生物活性导向的成分鉴定表明,脂肪酸和类固醇是负责非极性提取物生物活性的化合物。