Department of Physiology, Faculty of Biology, Complutense University of Madrid, Madrid, Spain.
Rejuvenation Res. 2010 Dec;13(6):665-74. doi: 10.1089/rej.2010.1049. Epub 2010 Sep 6.
In previous work we have observed that ovariectomy in rodents, a good model of mimicking human ovarian hormone loss, causes premature aging of the immune system. The prooxidative and inflammatory state that underlies the aging process is the base of that premature immunosenescence. It has been found that nutritional interventions with polyphenolic antioxidants constitute a good alternative to rejuvenate age-affected immune functions. In this study, we administered a diet supplemented with polyphenols (coming from soybean isoflavones and green tea) to sham-operated and ovariectomized mature mice for 15 weeks, until they reached a very old age. We have studied the effect of this supplementation on a broad range of parameters of immune function (in macrophages and lymphocytes) and oxidative stress (enzymatic and nonenzymatic antioxidant defences, oxidant compounds, and lipid peroxidation damage) in peritoneal leukocytes. The results showed that ovariectomy accelerates the age-related impairment of immune functions in very old mice as well as the oxidative and proinflammatory imbalance, and that the administration of soybean isoflavones and green tea improve the immune and redox state in these animals. Because the immune system is a good marker of health and a predictor of longevity, we suggest that an adequate nutritional treatment with polyphenols could be a highly recommended tool to fight against the detrimental effects of the lack of female sex hormones, through an improvement of the immune cell functions and redox state.
在之前的工作中，我们观察到，卵巢切除术（啮齿动物模型，可模拟人类卵巢激素丧失）导致免疫系统过早衰老。衰老过程中存在的促氧化和炎症状态是这种过早免疫衰老的基础。已经发现，用多酚抗氧化剂进行营养干预是恢复受年龄影响的免疫功能的一种很好的选择。在这项研究中，我们给假手术和卵巢切除的成熟小鼠喂食了 15 周富含多酚（来自大豆异黄酮和绿茶）的饮食，直到它们达到非常老的年龄。我们研究了这种补充对广泛的免疫功能参数（巨噬细胞和淋巴细胞）和氧化应激（酶和非酶抗氧化防御、氧化剂化合物和脂质过氧化损伤）的影响在腹膜白细胞中。结果表明，卵巢切除术加速了非常老年小鼠的免疫功能与氧化和促炎失衡相关的衰老损伤，而大豆异黄酮和绿茶的给药改善了这些动物的免疫和氧化还原状态。因为免疫系统是健康的良好标志物和长寿的预测因子，所以我们建议，用多酚进行适当的营养治疗可能是一种非常有用的工具，可以通过改善免疫细胞功能和氧化还原状态来对抗缺乏女性性激素的不利影响。
