Department of Physiology (Animal Physiology II), Faculty of Biological Sciences, Complutense University, c/Jose Antonio Novais no. 2, 28040, Madrid, Spain.
Eur J Nutr. 2011 Oct;50(7):563-73. doi: 10.1007/s00394-010-0163-2. Epub 2011 Jan 8.
To study the effect of diet supplementation with polyphenols on several functions suffering age-related changes, in peritoneal leucocytes from mature and old mice.
Five groups of female ICR mice were used. Four groups received a supplementation (20% wt/wt) of biscuits with different cereal fractions naturally rich in polyphenols (named CO49, CO50, CO52, CO53), containing different amounts of catechin, p-hydroxybenzoic acid, vanillic acid, p-coumaric acid, sinapic acid, ferulic acid, rutin and oryzanol. The control group received only standard maintenance diet. Peritoneal suspensions were obtained after 15 and 30 weeks of diet supplementation, when the age of the animals was 49 ± 2 (mature mice) and 64 ± 2 weeks (old mice), respectively. The functions analysed were: chemotaxis of macrophages and lymphocytes, phagocytosis of particles by macrophages, intracellular superoxide anion levels, lymphoproliferative response to mitogens (concanavalin A and lipopolysaccharide), interleukin-2 secretion and natural killer (NK) activity, as functions that decrease with age, and adherence of macrophages and lymphocytes and tumour necrosis factor-α secretion as functions with age-related increase.
The supplementation, in general, increased the functions that decrease with age and decreased those that increase with age. There were differences in the effects shown by the four kinds of biscuits depending on the function studied and the number of weeks of supplementation.
Since the immune system has been proposed as a good marker of health and predictor of longevity, diet supplementation with cereals naturally rich in polyphenols could be an important way for health preservation with age and reaching high longevity.
研究多酚饮食补充对成熟和老年小鼠腹腔白细胞中几种与年龄相关变化的功能的影响。
使用五组雌性 ICR 小鼠。四组接受不同谷物部分的饼干补充（20％重量/重量），这些饼干天然富含多酚（命名为 CO49、CO50、CO52、CO53），含有不同量的儿茶素、对羟基苯甲酸、香草酸、对香豆酸、芥子酸、阿魏酸、芦丁和谷维素。对照组仅接受标准维持饮食。在饮食补充 15 和 30 周后，即动物年龄分别为 49 ± 2（成熟小鼠）和 64 ± 2 周（老年小鼠）时，获得腹腔混悬液。分析的功能有：巨噬细胞和淋巴细胞的趋化性、巨噬细胞吞噬颗粒、细胞内超氧阴离子水平、丝裂原（刀豆蛋白 A 和脂多糖）刺激的淋巴细胞增殖反应、白细胞介素-2 分泌和自然杀伤（NK）活性，这些功能随着年龄的增长而降低，以及巨噬细胞和淋巴细胞的粘附和肿瘤坏死因子-α的分泌，这些功能随着年龄的增长而增加。
补充物通常会增加随年龄下降的功能，减少随年龄增加的功能。四种饼干的作用效果因研究的功能和补充的周数而异。
由于免疫系统被认为是健康的良好标志物和长寿的预测因子，因此，富含多酚的天然谷物饮食补充可能是随着年龄保持健康和达到高寿的重要途径。
