婴儿体内针对肠道病毒 71 型和柯萨奇病毒 A16 的母源中和抗体的动态变化。

Dynamic change of mother-source neutralizing antibodies against enterovirus 71 and coxsackievirus A16 in infants.

机构信息

2nd Division of Viral Vaccines, National Institute for Control of Pharmaceutical and Biological Products, Beijing 100050, China.

出版信息

Chin Med J (Engl). 2010 Jul;123(13):1679-84.

PMID:20819628

Abstract

BACKGROUND

Enterovirus 71 (EV71) and coxsackievirus A16 (Cox A16) are major causative agents for hand, foot and mouth disease (HFMD). Studies indicate that the frequent HFMD outbreaks result in a few hundreds children's death in China in recent years. The vaccine and other research for HFMD need to be developed urgently.

THE AIMS OF OUR STUDY WERE

to explore dynamic development of mother-source neutralizing antibodies against EV71 and Cox A16 in infants from Jiangsu Province, China, and to provide the fundamental data for further establishing of corresponding immunization course.

METHODS

Peripheral blood samples were collected from 133 of parturient women once immediately before delivery and their infants at two and seven months of age. Method of micro-dose cytopathogenic effect was used to measure neutralizing antibodies against EV71 and Cox A16, respectively.

RESULTS

Seropositive rates of anti-EV71 and anti-Cox A16 in prenatal women were 79.7% (106/133) and 92.5% (123/133), respectively; geometric mean titers (GMTs) were 29.0 and 61.9; 75.9% (101/133) prenatal women were both positive in anti-EV71 and anti-Cox A16; seropositive rates of anti-EV71 and anti-Cox A16 were 25.6% (34/133) and 38.3% (51/133) in infants at two months of age; GMTs were 12.3 and 18.0, respectively. GMTs of anti-EV71 were significantly higher for infants at seven months (82.6) compared with that at two months (P < 0.05), showing infants had inapparently infected by EV71 during two to seven months. Although only one offspring (0.75%) at seven months was found having anti-Cox A16 transfered from maternal, this observation suggested no maternal antibody may remain in infants at seven months.

CONCLUSIONS

The prevalence of EV71 and Cox A16 were relatively high in Jiangsu Province. Bivalent vaccine against both EV71 and Cox A16 should be developed, and the ideal time point for prime immunization for infants is around 2-5 months of age.

摘要

背景

肠道病毒 71 型（EV71）和柯萨奇病毒 A16 型（Cox A16）是手足口病（HFMD）的主要病原体。研究表明，近年来中国手足口病的频繁爆发导致了数百名儿童死亡。疫苗和其他手足口病的研究亟待开展。

我们研究的目的是

探索江苏省婴儿母源中和抗体针对 EV71 和 Cox A16 的动态发展，并为进一步建立相应的免疫接种程序提供基础数据。

方法

采集 133 名产妇分娩前及婴儿 2 个月和 7 个月时的外周血样本。采用微量细胞病变法检测 EV71 和 Cox A16 的中和抗体。

结果

产前妇女抗-EV71 和抗-Cox A16 的血清阳性率分别为 79.7%（106/133）和 92.5%（123/133），几何平均滴度（GMT）分别为 29.0 和 61.9；75.9%（101/133）的产前妇女抗-EV71 和抗-Cox A16 均为阳性；婴儿 2 个月时抗-EV71 和抗-Cox A16 的血清阳性率分别为 25.6%（34/133）和 38.3%（51/133），GMT 分别为 12.3 和 18.0。与 2 个月时相比，7 个月时婴儿抗-EV71 的 GMT 明显升高（P<0.05），提示婴儿在 2 至 7 个月期间可能已感染 EV71，但无明显症状。虽然只有一个婴儿（0.75%）在 7 个月时从母体转移到抗-Cox A16，但这一观察结果表明，7 个月大的婴儿体内可能没有母体抗体。