Martz W, Oellerich M, Schumann G
Institut für Klinische Chemie I, Medizinische Hochschule Hannover.
J Clin Chem Clin Biochem. 1990 Dec;28(12):951-3.
The determination of methotrexate on the aca clinical analyser (aca MTHO method) was evaluated and the method compared with a fluorescence polarisation immunoassay (FPIA) and a specific high-performance liquid chromatography (HPLC) procedure. The within series and the between days coefficients of variation of measurements in human pool serum spiked with methotrexate (0.07 mumol/l to 15 mumol/l) ranges from 11.7% to 2.5% and 14.0% to 5.8%, respectively. The calibration was found to be stable for 9 weeks. There was a good correlation between the results of the MTHO method when compared with FPIA and HPLC. At low methotrexate concentration, the results of HPLC were on average 11% and 14% lower than those obtained by the MTHO assay and FPIA respectively.
对aca临床分析仪上甲氨蝶呤的测定方法（aca MTHO法）进行了评估，并将该方法与荧光偏振免疫分析法（FPIA）和特定的高效液相色谱法（HPLC）进行了比较。在添加了甲氨蝶呤（0.07μmol/L至15μmol/L）的人混合血清中进行测量，其批内和批间变异系数分别为11.7%至2.5%和14.0%至5.8%。校准在9周内保持稳定。与FPIA和HPLC相比，MTHO法的结果具有良好的相关性。在低甲氨蝶呤浓度下，HPLC的结果分别比MTHO法和FPIA法获得的结果平均低11%和14%。
