The Jackson Laboratory, 600 Main Street, Bar Harbor, ME 04609, USA.
J Gerontol A Biol Sci Med Sci. 2010 Dec;65(12):1275-84. doi: 10.1093/gerona/glq155. Epub 2010 Sep 5.
We used a heterogeneous stock of mice-UM-HET3, the first generation offspring of CByB6F1/J and C3D2F1/J parents-to test effects of six antiaging treatments on life span. In the first report of diet restriction in a structured, segregating heterogeneous population, we observed essentially the same increases in mean and maximum life span as found in CByB6F1/J hybrid positive controls. We also report results of treatment with N-acetyl-L-cysteine started at 7 months, and aspirin, nitroflurbiprofen, 4-hydroxy phenyl N-tert-butyl nitrone, and nordihydroguaiaretic acid, all started at 16-18 months. Only male UM-HET3 mice receiving N-acetyl-L-cysteine had significantly increased life span, and this may have been due to treatment-related inadvertent diet restriction. The other agents had no significant effects on life span. The use of UM-HET3 mice helps assure that these results are not the result of unresponsiveness of a single genotype but that they more broadly represent laboratory mice.
我们使用了一组异质的小鼠种群 UM-HET3，即 CByB6F1/J 和 C3D2F1/J 父母的第一代后代，来测试六种抗衰老治疗方法对寿命的影响。这是首次在结构上分离的异质群体中进行饮食限制的报告，我们观察到与 CByB6F1/J 杂交阳性对照中发现的平均和最大寿命延长基本相同。我们还报告了从 7 个月开始用 N-乙酰-L-半胱氨酸、阿司匹林、硝基氟布布洛芬、4-羟基苯基-N-叔丁基硝酮和 Nordihydroguaiaretic 酸治疗的结果，所有这些药物均从 16-18 个月开始治疗。只有接受 N-乙酰-L-半胱氨酸治疗的雄性 UM-HET3 小鼠的寿命显著延长，这可能是由于治疗相关的意外饮食限制所致。其他药物对寿命没有显著影响。使用 UM-HET3 小鼠有助于确保这些结果不是由于单一基因型的不反应所致，而是更广泛地代表了实验室小鼠。
