College of Sciences, Shenyang Agricultural University, Shenyang, China.
Talanta. 2010 Jul 15;82(2):693-7. doi: 10.1016/j.talanta.2010.05.034. Epub 2010 May 21.
The purpose of this study was to establish a simple and sensitive analytical method for lysozyme using Plasmon Resonance Light-Scattering (PRLS) technique with Gold Nanoparticles (AuNPs) as the probe. Nanomolar level of lysozyme induced AuNPs aggregation with enhanced PRLS. For 1.4 nM citrate-capped AuNPs (13 nm in diameter), the linear range of the calibration curve was 15-50 nM with a detection limit of 13.1 nM for lysozyme. Six nanomolar lysozyme can produce an observable PRLS enhancement. Most potential interfering substances present in urine had a negligible effect on the determination. The interference from human serum albumin in the urinary sample can be reduced by precipitating the albumin with ethanol at pH 4.8-4.9. The 90.1-118.2% recovery was achieved for 8 individual lysozyme-spiked urinary samples. This simple and sensitive method for lysozyme does not require sample clean-up and AuNPs modification, thus provided an alternative for urinary lysozyme determination.
本研究旨在建立一种使用金纳米粒子（AuNPs）作为探针的简单灵敏的溶菌酶分析方法。等离子体共振光散射（PRLS）技术。纳米摩尔水平的溶菌酶诱导 AuNPs 聚集，增强 PRLS。对于 1.4 nM 的柠檬酸钠封端的 AuNPs（直径 13nm），校准曲线的线性范围为 15-50 nM，溶菌酶的检测限为 13.1 nM。6 纳摩尔的溶菌酶可以产生可观察到的 PRLS 增强。尿液中存在的大多数潜在干扰物质对测定的影响可以忽略不计。通过在 pH 4.8-4.9 下用乙醇沉淀白蛋白，可以减少尿液样品中人血清白蛋白的干扰。对于 8 个单独的溶菌酶加标尿液样品，回收率为 90.1-118.2%。该方法简单灵敏，不需要样品净化和 AuNPs 修饰，为尿液溶菌酶的测定提供了一种替代方法。
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