Department of Food and Environmental Sciences, University of Helsinki, P. O. Box 27, FIN-00014 Helsinki, Finland.
Anal Bioanal Chem. 2011 Feb;399(4):1467-72. doi: 10.1007/s00216-010-4160-3. Epub 2010 Sep 6.
Asymmetric flow field-flow fractionation (AsFlFFF) and high-performance size-exclusion chromatography (HPSEC) are techniques for separating and characterizing macromolecules; until now the latter is more utilized for analyzing polysaccharides. The demand for characterizing complex, high-molar-mass polysaccharides has raised interest in the use of AsFlFFF in analyzing polymeric carbohydrates in addition to HPSEC. In this paper, we compare the behavior of arabinoxylan aggregates present in aqueous solution in AsFlFFF and HPSEC and their effect on the obtained molecular characteristics (molar mass averages and size). Although the amount of aggregates in aqueous arabinoxylan solutions may be low, their role needs to be understood to avoid erroneous interpretations of AsFlFFF and HPSEC data. When these two separation systems were compared, AsFlFFF seemed to possess more separation power for the differentiation of aggregates from individual chains than HPSEC. To our knowledge, this is the first report on the characterization of xylans with AsFlFFF.
不对称流场流分离（AsFlFFF）和高效体积排阻色谱（HPSEC）是用于分离和表征生物大分子的技术；到目前为止，后者更多地用于分析多糖。对分析复杂、高摩尔质量多糖的需求，提高了在分析聚合碳水化合物时使用 AsFlFFF 的兴趣，除了 HPSEC 之外。在本文中，我们比较了水溶液中存在的阿拉伯木聚糖聚集体在 AsFlFFF 和 HPSEC 中的行为及其对获得的分子特性（摩尔质量平均值和尺寸）的影响。尽管水溶液中阿拉伯木聚糖聚集体的数量可能较低，但需要了解它们的作用，以避免对 AsFlFFF 和 HPSEC 数据的错误解释。当比较这两种分离系统时，AsFlFFF 似乎比 HPSEC 具有更强的分离能力，可将聚集体与单个链区分开来。据我们所知，这是首次使用 AsFlFFF 对木聚糖进行表征的报告。
