Department of Food and Environmental Sciences, P.O. Box 27, FIN-00014 University of Helsinki, Finland.
Carbohydr Polym. 2014 Jan;99:199-207. doi: 10.1016/j.carbpol.2013.08.021. Epub 2013 Aug 21.
Dilute solutions of various dextran standards, a high-molar mass (HMM) commercial dextran from Leuconostoc spp., and HMM dextrans isolated from Weissella confusa and Leuconostoc citreum were analyzed with high-performance size-exclusion chromatography (HPSEC), asymmetric flow field-flow fractionation (AsFlFFF), and diffusion-ordered NMR spectroscopy (DOSY). HPSEC analyses were performed in aqueous and dimethyl sulfoxide (DMSO) solutions, while only aqueous solutions were utilized in AsFlFFF and DOSY. The study showed that all methods were applicable to dextran analysis, but differences between the aqueous and DMSO-based solutions were obtained for HMM samples. These differences were attributed to the presence of aggregates in aqueous solution that were less prevalent in DMSO. The study showed that DOSY provides an estimate of the size of HMM dextrans, though calibration standards may be required for each experimental set-up. To our knowledge, this is the first study utilizing these three methods in analyzing HMM dextrans.
用高效体积排阻色谱法（HPSEC）、不对称流场流分离（AsFlFFF）和扩散排序 NMR 光谱学（DOSY）分析了各种葡聚糖标准品、来自肠膜明串珠菌的高分子量（HMM）商业葡聚糖和从魏斯氏菌和柠檬色明串珠菌分离的 HMM 葡聚糖的稀溶液。HPSEC 分析在水溶液和二甲基亚砜（DMSO）溶液中进行，而 AsFlFFF 和 DOSY 仅在水溶液中进行。研究表明，所有方法均适用于葡聚糖分析，但对于 HMM 样品，在水溶液和基于 DMSO 的溶液之间获得了差异。这些差异归因于在水溶液中存在聚集物，而在 DMSO 中则较少出现。该研究表明，DOSY 可提供 HMM 葡聚糖大小的估计值，但可能需要为每个实验设置校准标准。据我们所知，这是首次利用这三种方法分析 HMM 葡聚糖的研究。
