Ortega-Pierres G, Alonso R, Cervantes L, Montañez C
Department of Genetics and Molecular Biology, Centro de Investigacion y de Estudios Avanzados IPN, Mexico D.F.
Mol Microbiol. 1990 Nov;4(11):1985-91. doi: 10.1111/j.1365-2958.1990.tb02048.x.
Total DNA was isolated from the parasitic protozoan Giardia lamblia and separated into two distinct populations of different densities by centrifugation through CsCl gradients containing Hoechst dye 33258. The two populations obtained were characterized by restriction enzyme analysis and nucleic acid hybridization. The less-dense population contains non-repetitive DNA and may encode mainly structural genes, such as those for alpha- and beta-tubulin. Digestion of the DNA with several restriction endonucleases showed that the denser band was composed of a 5.5 kb unit which contains the G. lamblia ribosomal RNA cistron in tandem repeated organization.
从寄生原生动物蓝氏贾第鞭毛虫中分离出总DNA，并通过含有Hoechst染料33258的氯化铯梯度离心将其分离成两个不同密度的群体。通过限制性酶切分析和核酸杂交对获得的两个群体进行了表征。密度较小的群体包含非重复DNA，可能主要编码结构基因，如α-和β-微管蛋白的基因。用几种限制性内切酶消化DNA表明，密度较大的条带由一个5.5 kb的单元组成，该单元以串联重复的形式包含蓝氏贾第鞭毛虫核糖体RNA顺反子。
